02104nam a2200217Ia 4500 TDMU_31329 210410s9999 xx 000 0 und d 004.65 PH102 Phage display a laboratory manual by Carlos F. Barbas III ... [et al.] Cold Spring Harbor, NY Cold Spring Harbor Laboratory Press 2001 1 v. in various pagings Includes bibliographical references and index; Filamentous phage biology -- Phage-display vectors -- Antibody libraries -- Peptide libraries -- Functional domains and scaffolds -- Gene fragment libraries and genomic and cDNA expression cloning -- Overview : Amplification and antibody genes -- Generation of antibody libraries : PCR amplification and assembly of light- and heavy-chain coding sequences -- Selection from antibody libraries -- Analysis of selected antibodies -- Production and purfification of Fab and scFv -- Antibody engineering -- Overview : Peptide libraries -- General phage methods -- Screening peptide libraries -- Analysis of phage-borne peptides -- Construction and use of pIII-displayed peptide libraries -- Construction and selection from gene fragment phage-display expression libraries -- Construction and selection from cDNA phage-display expression libraries -- In vivo selection of phage-display libraries -- Cell surface selection and analysis of monoclonal antibodies from phage libraries Affinity chromatography Laboratory manuals; Bacteriophages Laboratory manuals; Microbial biotechnology Laboratory manuals; Peptides Biotechnology Laboratory manuals; Proteins Biotechnology Laboratory manuals; Sắc ký ái lực Hướng dẫn phòng thí nghiệm; Thực khuẩn thể ; Công nghệ sinh học vi sinh vật; Chất peptides Công nghệ sinh học Barbas, Carlos F http://lrc.tdmu.edu.vn/opac/search/detail.asp?aID=2&ID=31329 Trung tâm Học liệu Trường Đại học Thủ Dầu Một