Assessment of genetic stability and photoreceptor gene expression (CmPHYA and CmPHYB) of Chrysanthemum indicium plantlets culture on medium containing molybdenum trioxide nanoparticles

This study investigated the effects of molybdenum trioxide nanoparticles (MoO₃NPs) on the rooting, genetic stability, photosynthesis-related gene expression, and nursery-stage growth of Chrysanthemum indicum. The rooting stage analysis revealed that the 6.4 µg/L MoO₃NPs treatment significantly en...

وصف كامل

محفوظ في:
التفاصيل البيبلوغرافية
المؤلفون الرئيسيون: Tan Nhut Duong, Le Ha Nguyen Phan, Thi Nhu Mai Nguyen, Manh Cuong Do, Hai Dang Hoang, Quoc Luan Vu, Hoang Thanh Tung, Hoàng, Thị Như Phương, Hong Thien Van, Quang Vinh Nguyen, Bich Ngoc Pham, Hoang Ha Chu
التنسيق: Journal article
اللغة:English
منشور في: 2025
الموضوعات:
الوصول للمادة أونلاين:https://scholar.dlu.edu.vn/handle/123456789/4724
الوسوم: إضافة وسم
لا توجد وسوم, كن أول من يضع وسما على هذه التسجيلة!
Thư viện lưu trữ: Thư viện Trường Đại học Đà Lạt
الوصف
الملخص:This study investigated the effects of molybdenum trioxide nanoparticles (MoO₃NPs) on the rooting, genetic stability, photosynthesis-related gene expression, and nursery-stage growth of Chrysanthemum indicum. The rooting stage analysis revealed that the 6.4 µg/L MoO₃NPs treatment significantly enhanced rooting rate, plantlet height, leaf number, root number, root length, SPAD value, and fresh weight compared to control treatments. ITS sequence analysis confirmed that MoO₃NPs did not induce mutations or genetic instability, with the ITS sequences of treated plantlets showing 100% similarity with those in the NCBI database and control samples. MoO₃NPs supplementation also upregulated the expression of CmPHYA and CmPHYB, critical genes encoding photoreceptors involved in red and far-red light perception, enhancing photosynthetic efficiency. At the nursery stage, plantlets derived from MoO₃NPs-supplemented media demonstrated superior growth parameters, including plant height, leaf and root numbers, fresh and dry weight, and SPAD value. These findings underscore the potential of MoO₃NPs to improve plantlet development, photosynthetic performance, and nutrient absorption, thereby highlighting their role in sustainable plant production systems.