Molecular cloning, expression, characterization of a functional single-chain Fv antibody to the mycotoxin zearalenone /

Míreanna Comhchosúla

• A sensitive and inexpensive yeast bioassay for the mycotoxin zearalenone and other compounds with estrogenic activity /
• Procaryotic expression of single-chain variable-fragment (scfv) antibodies : Secretion in L-form cells of proteus mirabilis leads to active product and overcomes the limitations of periplasmic expression in escherichia coli /
• One hundred seventy-fold increase in excretion of an FV fragment-tumor necrosis factor alpha fusion protein (sFV/TNF-) from escherichia coli caused by the synergistic effects of glycine and triton X-100 /
• Stability of a single-chain Fv antibody fragment when exposed to a high shear environment combined with air-liquid interfaces /
  le: Harrison, J. S.
• Engineering of a single-chain variable-fragment (scFv) antibody specific for the stolbur phytoplasma (Mollicute) and its expression in escherichia coli and tobacco plants /
  le: Gall, Fabrice Le.