A new sensitive, whole-cell hybridization technique for detection of bacteria involving a biotinylated oligonucleotide probe targeting rRNA and tyramide signal amplification /

Míreanna Comhchosúla

• Quantitative assessment of picoeukaryotes in the natural environment by using taxon-specific oligonucleotide probes in association with tyramide signal amplification-fluorescence in situ hybridization and flow cytometry /
• Improved sensitivity of whole-cell hybridization by the combination of horseradish peroxidase-labeled oligonucleotides and tyramide signal amplification /
• Flow cytometric analysis of the in situ accessibility of escherichia coli 16s rrna for fluorescently labeled oligonucleotide probes /
• Characterization of the cricket hindgut microbiota with fluorescently labeled rRNA-targeted oligonucleotide probes /
• Optimization strategies for DNA microarray-based detection of bacteria with 16S rRNA-targeting oligonucleotide probes /
  le: Peplies, Jorg.