Affinity chromatography with collapsibly tethered ligands /

Eitemau Tebyg

• A smart microfluidic affinity chromatography matrix composed of poly(n-isopropylacrylamide)-coated beads /
• Directed immobilization of peptide ligands to accessible pore sites by conjugation with a placeholder molecule /
• High resolution chromatography :
  Cyhoeddwyd: (1999)
• Improved beta-elimination-based affinity purification strategy for enrichment of phosphopeptides /
  gan: McLachlin, Derek T.
• Characterization of protein phosphorylation by mass spectrometry using immobilized metal ion affinity chromatography with on-resin beta-elimination and michael addition /