發 展 Bac-to-Bac 之 桿 狀 病 毒 雙 效 表 現 載 體 (Development of bicistronic baculovirus expression system vector using BAC-to-BAC system)

發 展 Bac-to-Bac 之 桿 狀 病 毒 雙 效 表 現 載 體 (Development of bicistronic baculovirus expression system vector using BAC-to-BAC system)

Baculoviruses are one of the most studies insect viruses both in basic virology research and in biotechnology applications. This insect viruses based baculovirus expression vector system (BEVS) is an important tool for molecular biology and widely used in insect cells. Development of a shuttle bacul...

Mô tả đầy đủ

Đã lưu trong:
Chi tiết về thư mục
Tác giả chính: Nguyen Hoang Xuan Thao
Tác giả khác: 吳宗遠 博士 (người hướng dẫn khoa học)
Ngôn ngữ:vie
Được phát hành: 中原大學 2022
Truy cập trực tuyến:https://opac.tvu.edu.vn/pages/opac/wpid-detailbib-id-39031.html
Các nhãn: Thêm thẻ
Không có thẻ, Là người đầu tiên thẻ bản ghi này!
Thư viện lưu trữ: Trung tâm Học liệu – Phát triển Dạy và Học, Trường Đại học Trà Vinh
id https:--opac.tvu.edu.vn:9090-api-oai:39031
record_format dspace
spelling https:--opac.tvu.edu.vn:9090-api-oai:390312022-02-10T02:56:43Z 發 展 Bac-to-Bac 之 桿 狀 病 毒 雙 效 表 現 載 體 (Development of bicistronic baculovirus expression system vector using BAC-to-BAC system) Nguyen Hoang Xuan Thao Baculoviruses are one of the most studies insect viruses both in basic virology research and in biotechnology applications. This insect viruses based baculovirus expression vector system (BEVS) is an important tool for molecular biology and widely used in insect cells. Development of a shuttle baculovirus expression vector which can function in both the mammalian cell as well as in insect cell would have a significant advantages. We had cooperated an internal ribosome entry site (IRES) in to the baculovirus genomes and generated a bicistronic baculoviruses expression vector that can express two genes simultaneously. In this project, we attempt to construct a baculovirus vector with an IRES based bicistronic expression vector which using Bac-to-Bac system. The Bac-toBac system bring more benefits than the traditional methods using homologous recombination in insect cells. Bac-to-Bac plasmids: pFastBac1-Polh-MCS-Lir-DsRed2 had been successfully generated. Such IRES-based Bac-to-Bac BEVS will facilitate rapid and efficient generation of recombinant baculoviruses: time saving expression bacmid, one-step purification and amplification, rapid and simultaneous isolation of multiple recombinant viruses as well as titer determination 中原大學 吳宗遠 博士 (người hướng dẫn khoa học) 2022-02-10T02:56:43Z pdf https://opac.tvu.edu.vn/pages/opac/wpid-detailbib-id-39031.html vie
institution Trung tâm Học liệu – Phát triển Dạy và Học, Trường Đại học Trà Vinh
collection Thư viện số
language vie
description Baculoviruses are one of the most studies insect viruses both in basic virology research and in biotechnology applications. This insect viruses based baculovirus expression vector system (BEVS) is an important tool for molecular biology and widely used in insect cells. Development of a shuttle baculovirus expression vector which can function in both the mammalian cell as well as in insect cell would have a significant advantages. We had cooperated an internal ribosome entry site (IRES) in to the baculovirus genomes and generated a bicistronic baculoviruses expression vector that can express two genes simultaneously. In this project, we attempt to construct a baculovirus vector with an IRES based bicistronic expression vector which using Bac-to-Bac system. The Bac-toBac system bring more benefits than the traditional methods using homologous recombination in insect cells. Bac-to-Bac plasmids: pFastBac1-Polh-MCS-Lir-DsRed2 had been successfully generated. Such IRES-based Bac-to-Bac BEVS will facilitate rapid and efficient generation of recombinant baculoviruses: time saving expression bacmid, one-step purification and amplification, rapid and simultaneous isolation of multiple recombinant viruses as well as titer determination
author2 吳宗遠 博士 (người hướng dẫn khoa học)
author_facet 吳宗遠 博士 (người hướng dẫn khoa học)
Nguyen Hoang Xuan Thao
author Nguyen Hoang Xuan Thao
spellingShingle Nguyen Hoang Xuan Thao
發 展 Bac-to-Bac 之 桿 狀 病 毒 雙 效 表 現 載 體 (Development of bicistronic baculovirus expression system vector using BAC-to-BAC system)
author_sort Nguyen Hoang Xuan Thao
title 發 展 Bac-to-Bac 之 桿 狀 病 毒 雙 效 表 現 載 體 (Development of bicistronic baculovirus expression system vector using BAC-to-BAC system)
title_short 發 展 Bac-to-Bac 之 桿 狀 病 毒 雙 效 表 現 載 體 (Development of bicistronic baculovirus expression system vector using BAC-to-BAC system)
title_full 發 展 Bac-to-Bac 之 桿 狀 病 毒 雙 效 表 現 載 體 (Development of bicistronic baculovirus expression system vector using BAC-to-BAC system)
title_fullStr 發 展 Bac-to-Bac 之 桿 狀 病 毒 雙 效 表 現 載 體 (Development of bicistronic baculovirus expression system vector using BAC-to-BAC system)
title_full_unstemmed 發 展 Bac-to-Bac 之 桿 狀 病 毒 雙 效 表 現 載 體 (Development of bicistronic baculovirus expression system vector using BAC-to-BAC system)
title_sort 發 展 bac-to-bac 之 桿 狀 病 毒 雙 效 表 現 載 體 (development of bicistronic baculovirus expression system vector using bac-to-bac system)
publisher 中原大學
publishDate 2022
url https://opac.tvu.edu.vn/pages/opac/wpid-detailbib-id-39031.html
_version_ 1812601658840973312

Những quyển sách tương tự