Heterologous Protein Production in CHO Cells: Methods and Protocols
This book includes reviews and protocols for genetic manipulation of CHO cells for recombinant protein production, including “difficult-to-express” therapeutics. A method is also included on the use of the recently described genome editing tool, CRISPR/Cas9, and how this can be applied to CHO cells....
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| Tác giả chính: | |
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| Định dạng: | Sách |
| Ngôn ngữ: | en_US |
| Được phát hành: |
Humana Press
2020
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| Những chủ đề: | |
| Truy cập trực tuyến: | http://thuvien.ued.udn.vn/handle/TVDHSPDN_123456789/57300 |
| Các nhãn: |
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| Thư viện lưu trữ: | Trung tâm Học liệu và E-Learning, Trường Đại học Sư phạm – Đại học Đà Nẵng |
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| Tóm tắt: | This book includes reviews and protocols for genetic manipulation of CHO cells for recombinant protein production, including “difficult-to-express” therapeutics. A method is also included on the use of the recently described genome editing tool, CRISPR/Cas9, and how this can be applied to CHO cells. The book also includes a review and protocols for characterization of CHO cells using ‘omic approaches and how these methods can be used to improve efficiency of recombinant protein production during cell line development. |
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