Variation in genome size, cell and nucleus volume, chromosome number and rDNA loci among duckweeds
Duckweeds are small, free-floating, largely asexual and highly neotenous organisms. They display the most rapid growth among flowering plants and are of growing interest in aquaculture and genome biology. Genomic and chromosomal data are still rare. Applying flow-cytometric genome size measuremen...
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2022
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| Truy cập trực tuyến: | http://scholar.dlu.edu.vn/handle/123456789/1027 |
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Thư viện Trường Đại học Đà Lạt |
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English |
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Duckweeds are small, free-floating, largely asexual and highly neotenous organisms. They display
the most rapid growth among flowering plants and are of growing interest in aquaculture and
genome biology. Genomic and chromosomal data are still rare. Applying flow-cytometric genome
size measurement, microscopic determination of frond, cell and nucleus morphology, as well as
fluorescence in situ hybridization (FISH) for localization of ribosomal DNA (rDNA), we compared eleven
species, representative for the five duckweed genera to search for potential correlations between
genome size, cell and nuclei volume, simplified body architecture (neoteny), chromosome numbers and
rDNA loci. We found a ~14-fold genome size variation (from 160 to 2203 Mbp), considerable differences
in frond size and shape, highly variable guard cell and nucleus size, chromosome number (from 2n = 36
to 82) and number of 5S and 45S rDNA loci. In general, genome size is positively correlated with guard
cell and nucleus volume (p < 0.001) and with the neoteny level and inversely with the frond size. In
individual cases these correlations could be blurred for instance by particular body and cell structures
which seem to be linked to specific floating styles. Chromosome number and rDNA loci variation
between the tested species was independent of the genome size. We could not confirm previously
reported intraspecific variation of chromosome numbers between individual clones of the genera
Spirodela and Landoltia. |
| format |
Journal article |
| author |
Phuong T. N. Hoang Veit Schubert Armin Meister Jörg Fuchs Ingo Schubert |
| spellingShingle |
Phuong T. N. Hoang Veit Schubert Armin Meister Jörg Fuchs Ingo Schubert Variation in genome size, cell and nucleus volume, chromosome number and rDNA loci among duckweeds |
| author_facet |
Phuong T. N. Hoang Veit Schubert Armin Meister Jörg Fuchs Ingo Schubert |
| author_sort |
Phuong T. N. Hoang |
| title |
Variation in genome size, cell and nucleus volume, chromosome number and rDNA loci among duckweeds |
| title_short |
Variation in genome size, cell and nucleus volume, chromosome number and rDNA loci among duckweeds |
| title_full |
Variation in genome size, cell and nucleus volume, chromosome number and rDNA loci among duckweeds |
| title_fullStr |
Variation in genome size, cell and nucleus volume, chromosome number and rDNA loci among duckweeds |
| title_full_unstemmed |
Variation in genome size, cell and nucleus volume, chromosome number and rDNA loci among duckweeds |
| title_sort |
variation in genome size, cell and nucleus volume, chromosome number and rdna loci among duckweeds |
| publishDate |
2022 |
| url |
http://scholar.dlu.edu.vn/handle/123456789/1027 |
| _version_ |
1768305920626917376 |
| spelling |
oai:scholar.dlu.edu.vn:123456789-10272022-11-08T01:25:11Z Variation in genome size, cell and nucleus volume, chromosome number and rDNA loci among duckweeds Phuong T. N. Hoang Veit Schubert Armin Meister Jörg Fuchs Ingo Schubert Duckweeds are small, free-floating, largely asexual and highly neotenous organisms. They display the most rapid growth among flowering plants and are of growing interest in aquaculture and genome biology. Genomic and chromosomal data are still rare. Applying flow-cytometric genome size measurement, microscopic determination of frond, cell and nucleus morphology, as well as fluorescence in situ hybridization (FISH) for localization of ribosomal DNA (rDNA), we compared eleven species, representative for the five duckweed genera to search for potential correlations between genome size, cell and nuclei volume, simplified body architecture (neoteny), chromosome numbers and rDNA loci. We found a ~14-fold genome size variation (from 160 to 2203 Mbp), considerable differences in frond size and shape, highly variable guard cell and nucleus size, chromosome number (from 2n = 36 to 82) and number of 5S and 45S rDNA loci. In general, genome size is positively correlated with guard cell and nucleus volume (p < 0.001) and with the neoteny level and inversely with the frond size. In individual cases these correlations could be blurred for instance by particular body and cell structures which seem to be linked to specific floating styles. Chromosome number and rDNA loci variation between the tested species was independent of the genome size. 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A., Baldini, A. & Reeders, S. T. Improved telomere detection using a telomere repeat probe (TTAGGG)n generated by PCR. Nucleic Acids Res 19, 4780 (1991). 47. Hoang, P. T. N. & Schubert, I. Reconstruction of chromosome rearrangements between the two most ancestral duckweed species Spirodela polyrhiza and S. intermedia. Chromosoma 126, 729–739, https://doi.org/10.1007/s00412-017-0636-7 (2017). 48. Lysak, M. A. et al. Mechanisms of chromosome number reduction in Arabidopsis thaliana and related Brassicaceae species. Proc Natl Acad Sci USA 103, 5224–5229, https://doi.org/10.1073/pnas.0510791103 (2006). 49. Weisshart, K., Fuchs, J. & Schubert, V. Structured Illumination Microscopy (SIM) and Photoactivated Localization Microscopy (PALM) to Analyze the Abundance and Distribution of RNA Polymerase |