Limitation of current probe design for oligo-cross-FISH, exemplified by chromosome evolution studies in duckweeds
Duckweeds represent a small, free-floating aquatic family (Lemnaceae) of the monocot order Alismatales with the fastest growth rate among flowering plants. They comprise five genera (Spirodela, Landoltia, Lemna, Wolffiella, and Wolffia) varying in genome size and chromosome number. Spirodela polyr...
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| Định dạng: | Research article |
| Ngôn ngữ: | English |
| Được phát hành: |
2022
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| Những chủ đề: | |
| Truy cập trực tuyến: | http://scholar.dlu.edu.vn/handle/123456789/1030 |
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| Thư viện lưu trữ: | Thư viện Trường Đại học Đà Lạt |
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| Tóm tắt: | Duckweeds represent a small, free-floating aquatic family (Lemnaceae) of the monocot order Alismatales with the fastest growth
rate among flowering plants. They comprise five genera (Spirodela, Landoltia, Lemna, Wolffiella, and Wolffia) varying in
genome size and chromosome number. Spirodela polyrhiza had the first sequenced duckweed genome. Cytogenetic maps are
available for both species of the genus Spirodela (S. polyrhiza and S. intermedia). However, elucidation of chromosome
homeology and evolutionary chromosome rearrangements by cross-FISH using Spirodela BAC probes to species of other
duckweed genera has not been successful so far. We investigated the potential of chromosome-specific oligo-FISH probes to
address these topics. We designed oligo-FISH probes specific for one S. intermedia and one S. polyrhiza chromosome (Fig. 1a).
Our results show that these oligo-probes cross-hybridize with the homeologous regions of the other congeneric species, but are
not suitable to uncover chromosomal homeology across duckweeds genera. This is most likely due to too low sequence similarity
between the investigated genera and/or too low probe density on the target genomes. Finally, we suggest genus-specific design of
oligo-probes to elucidate chromosome evolution across duckweed genera. |
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