Silver nanoparticles improved explant disinfection, in vitro growth, runner formation and limited ethylene accumulation during micropropagation of strawberry (Fragaria × ananassa)
One of the common problems in strawberry (Fragaria × ananassa) micropropagation is the vitrification phenomenon (succulent plantlets, brittle stems, yellow leaves, etc.) leading to the reduction of plantlets quality and low survival rate in the greenhouse. In this study, the effects of silver nano...
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| Ngôn ngữ: | English |
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2022
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| Truy cập trực tuyến: | https://scholar.dlu.edu.vn/handle/123456789/1031 |
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| Thư viện lưu trữ: | Thư viện Trường Đại học Đà Lạt |
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oai:scholar.dlu.edu.vn:123456789-1031 |
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Thư viện Trường Đại học Đà Lạt |
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| language |
English |
| topic |
Ethylene accumulation · Fragaria × ananassa · Runner · Silver nanoparticles · Vitrification phenomenon |
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Ethylene accumulation · Fragaria × ananassa · Runner · Silver nanoparticles · Vitrification phenomenon Hoàng, Thanh Tùng Hoàng, Thị Như Phương Nguyễn, Bá Nam Silver nanoparticles improved explant disinfection, in vitro growth, runner formation and limited ethylene accumulation during micropropagation of strawberry (Fragaria × ananassa) |
| description |
One of the common problems in strawberry (Fragaria × ananassa) micropropagation is the vitrification phenomenon (succulent
plantlets, brittle stems, yellow leaves, etc.) leading to the reduction of plantlets quality and low survival rate in the
greenhouse. In this study, the effects of silver nanoparticles (AgNPs) on explant disinfection, in vitro growth (shoot multiplication,
and root formation), runner formation as well as ethylene accumulation during micropropagation of strawberry were
investigated. The results showed that leaf explants treated with 200 mg/L AgNPs solution for 20 min was more effective in
explant disinfection and shoot regeneration than using 1 g/L HgCl2.
In addition, AgNPs stimulated the growth of shoot and
plantlet and as well shortened the duration of root formation (4 days) as compared to those in control without AgNPs during
micropropagation. Besides, AgNPs reduced the ethylene gas accumulation in the culture’s vessels of shoots (0.66 ppm) and
plants (0.06 ppm) compared to controls (1.77 ppm; 0.15 ppm; respectively). Moreover, AgNPs combination with culture
period (5; 10 or 15 days) effect root formation stage and acclimatization in the greenhouse. The plantlets that cultured on
MS medium supplemethed with 0.5 mg/L AgNPs during 10 days showed higher survival rate (93.33%) after 15 days as well
as runner formation per plant (8.00 runners) after 60 days in greenhouse than those in control. |
| format |
Journal article |
| author |
Hoàng, Thanh Tùng Hoàng, Thị Như Phương Nguyễn, Bá Nam |
| author_facet |
Hoàng, Thanh Tùng Hoàng, Thị Như Phương Nguyễn, Bá Nam |
| author_sort |
Hoàng, Thanh Tùng |
| title |
Silver nanoparticles improved explant disinfection, in vitro growth, runner formation and limited ethylene accumulation during micropropagation of strawberry (Fragaria × ananassa) |
| title_short |
Silver nanoparticles improved explant disinfection, in vitro growth, runner formation and limited ethylene accumulation during micropropagation of strawberry (Fragaria × ananassa) |
| title_full |
Silver nanoparticles improved explant disinfection, in vitro growth, runner formation and limited ethylene accumulation during micropropagation of strawberry (Fragaria × ananassa) |
| title_fullStr |
Silver nanoparticles improved explant disinfection, in vitro growth, runner formation and limited ethylene accumulation during micropropagation of strawberry (Fragaria × ananassa) |
| title_full_unstemmed |
Silver nanoparticles improved explant disinfection, in vitro growth, runner formation and limited ethylene accumulation during micropropagation of strawberry (Fragaria × ananassa) |
| title_sort |
silver nanoparticles improved explant disinfection, in vitro growth, runner formation and limited ethylene accumulation during micropropagation of strawberry (fragaria × ananassa) |
| publishDate |
2022 |
| url |
https://scholar.dlu.edu.vn/handle/123456789/1031 |
| _version_ |
1783866396057272320 |
| spelling |
oai:scholar.dlu.edu.vn:123456789-10312023-11-15T04:49:37Z Silver nanoparticles improved explant disinfection, in vitro growth, runner formation and limited ethylene accumulation during micropropagation of strawberry (Fragaria × ananassa) Hoàng, Thanh Tùng Hoàng, Thị Như Phương Nguyễn, Bá Nam Ethylene accumulation · Fragaria × ananassa · Runner · Silver nanoparticles · Vitrification phenomenon One of the common problems in strawberry (Fragaria × ananassa) micropropagation is the vitrification phenomenon (succulent plantlets, brittle stems, yellow leaves, etc.) leading to the reduction of plantlets quality and low survival rate in the greenhouse. In this study, the effects of silver nanoparticles (AgNPs) on explant disinfection, in vitro growth (shoot multiplication, and root formation), runner formation as well as ethylene accumulation during micropropagation of strawberry were investigated. The results showed that leaf explants treated with 200 mg/L AgNPs solution for 20 min was more effective in explant disinfection and shoot regeneration than using 1 g/L HgCl2. In addition, AgNPs stimulated the growth of shoot and plantlet and as well shortened the duration of root formation (4 days) as compared to those in control without AgNPs during micropropagation. Besides, AgNPs reduced the ethylene gas accumulation in the culture’s vessels of shoots (0.66 ppm) and plants (0.06 ppm) compared to controls (1.77 ppm; 0.15 ppm; respectively). Moreover, AgNPs combination with culture period (5; 10 or 15 days) effect root formation stage and acclimatization in the greenhouse. The plantlets that cultured on MS medium supplemethed with 0.5 mg/L AgNPs during 10 days showed higher survival rate (93.33%) after 15 days as well as runner formation per plant (8.00 runners) after 60 days in greenhouse than those in control. 2022-09-15T11:47:27Z 2022-09-15T11:47:27Z 2021 Journal article Bài báo đăng trên tạp chí thuộc ISI, bao gồm book chapter https://scholar.dlu.edu.vn/handle/123456789/1031 10.1007/s11240-021-02015-4 en PCTOC Parzymies M, Pudelska K, Poniewozik M (2019) The use of nano-silver for disinfection of Pennisetum alopecuroides plant material for tissue culture. 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