Paving the way for large-scale micropropagation of Juglans 3 intermedia using genetically identified hybrid seed
This study provides a basic tool for the production of a multiclonal variety of Juglans nigra 9 J. regia hybrids (J. 9 intermedia), including optimized in vitro propagation methods and DNA-based techniques for the identification of hybrid seeds. Following identification of hybrid seed material usi...
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Springer Science+Business Media Dordrecht
2022
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oai:scholar.dlu.edu.vn:123456789-1160 |
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Thư viện Trường Đại học Đà Lạt |
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English |
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Juglans hybrids DNA fingerprinting In vitro propagation Shoot proliferation Rugini medium Rooting |
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Juglans hybrids DNA fingerprinting In vitro propagation Shoot proliferation Rugini medium Rooting Phạm, Ngọc Tuân A. Meier-Dinkel A. M. Ho¨ltken I. Wenzlitschke T. Winkelmann Paving the way for large-scale micropropagation of Juglans 3 intermedia using genetically identified hybrid seed |
| description |
This study provides a basic tool for the production of a multiclonal variety of Juglans nigra 9 J. regia
hybrids (J. 9 intermedia), including optimized in vitro propagation methods and DNA-based techniques for the
identification of hybrid seeds. Following identification of hybrid seed material using DNA markers embryo axes dissected from mature nuts were used as primary explants for establishing shoot cultures. The growth of shoot cultures of four hybrid genotypes was compared on two different basal media (Rugini and DKW) with three concentrations (2.2; 4.4; and 8.8 lM) of 6-benzylaminopurine (BA), and three gelling agents (Oxoid agar, Kobe agar, Gelrite). Three indole-3-butyric acid (IBA) concentrations (4, 12, and 20 lM) were compared for root induction, as well as three media for root expression. In general, the best combination of shoot elongation and production of new axillary shoots was achieved with 4.4 lM BA and 0.2 lM IBA in Rugini and in DKW medium. However, shoot elongation in most genotypes was favored when 2.2 lM BA and 0.2 lM IBA was used, in both, DKW and Rugini medium. The optimal gelling agent for Juglans hybrid shoot cultures was Kobe agar in Rugini medium. Highest rooting percentages were obtained on DKW medium with 12 lM IBA with 5 days in darkness followed by root expression under light on a mixture of gelified � DKW medium and vermiculite. During acclimatization, more than 75 % of the plantlets continued to grow vigorously. |
| format |
Journal article |
| author |
Phạm, Ngọc Tuân A. Meier-Dinkel A. M. Ho¨ltken I. Wenzlitschke T. Winkelmann |
| author_facet |
Phạm, Ngọc Tuân A. Meier-Dinkel A. M. Ho¨ltken I. Wenzlitschke T. Winkelmann |
| author_sort |
Phạm, Ngọc Tuân |
| title |
Paving the way for large-scale micropropagation of Juglans 3 intermedia using genetically identified hybrid seed |
| title_short |
Paving the way for large-scale micropropagation of Juglans 3 intermedia using genetically identified hybrid seed |
| title_full |
Paving the way for large-scale micropropagation of Juglans 3 intermedia using genetically identified hybrid seed |
| title_fullStr |
Paving the way for large-scale micropropagation of Juglans 3 intermedia using genetically identified hybrid seed |
| title_full_unstemmed |
Paving the way for large-scale micropropagation of Juglans 3 intermedia using genetically identified hybrid seed |
| title_sort |
paving the way for large-scale micropropagation of juglans 3 intermedia using genetically identified hybrid seed |
| publisher |
Springer Science+Business Media Dordrecht |
| publishDate |
2022 |
| url |
http://scholar.dlu.edu.vn/handle/123456789/1160 |
| _version_ |
1768305951615483904 |
| spelling |
oai:scholar.dlu.edu.vn:123456789-11602022-10-03T08:58:26Z Paving the way for large-scale micropropagation of Juglans 3 intermedia using genetically identified hybrid seed Phạm, Ngọc Tuân A. Meier-Dinkel A. M. Ho¨ltken I. Wenzlitschke T. Winkelmann Juglans hybrids DNA fingerprinting In vitro propagation Shoot proliferation Rugini medium Rooting This study provides a basic tool for the production of a multiclonal variety of Juglans nigra 9 J. regia hybrids (J. 9 intermedia), including optimized in vitro propagation methods and DNA-based techniques for the identification of hybrid seeds. Following identification of hybrid seed material using DNA markers embryo axes dissected from mature nuts were used as primary explants for establishing shoot cultures. The growth of shoot cultures of four hybrid genotypes was compared on two different basal media (Rugini and DKW) with three concentrations (2.2; 4.4; and 8.8 lM) of 6-benzylaminopurine (BA), and three gelling agents (Oxoid agar, Kobe agar, Gelrite). Three indole-3-butyric acid (IBA) concentrations (4, 12, and 20 lM) were compared for root induction, as well as three media for root expression. In general, the best combination of shoot elongation and production of new axillary shoots was achieved with 4.4 lM BA and 0.2 lM IBA in Rugini and in DKW medium. However, shoot elongation in most genotypes was favored when 2.2 lM BA and 0.2 lM IBA was used, in both, DKW and Rugini medium. The optimal gelling agent for Juglans hybrid shoot cultures was Kobe agar in Rugini medium. Highest rooting percentages were obtained on DKW medium with 12 lM IBA with 5 days in darkness followed by root expression under light on a mixture of gelified � DKW medium and vermiculite. 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Mol Ecol 14(8):2611–2620. doi:10.1111/j. 1365-294x.2005.02553.x Fady B, Ducci F, Aleta N, Becquey J, Vazquez RD, Lopez FF, Rumpf H (2003) Walnut demonstrates strong genetic variability for adaptive and wood quality traits in a network of juvenile field tests across Europe. New For 25(3):211–225. doi:10.1023/A: 1022939609548 Fornari B, Malvolti ME, Taurchini D, Fineschi S, Beritognolo I, Maccaglia E, Cannata F (1999) Isozyme and organellar DNA analysis of genetic diversity in natural/naturalised european and asiatic walnut (Juglans regia L.) populations. In: 4th interna- tional walnut symposium, vol 544, pp 167–178. doi:10.17660/ actahortic.2001.544.2 Gamborg OL, Miller R, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50(1):151–158. doi:10.1016/0014-4827(68)90403-5 Gebhardt K (1985) Development of a sterile cultivation system for rooting of shoot tip cultures (red raspberries) in duroplast foam. 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