Factors affecting shoot multiplication and rooting of walnut (Juglans regia L.) in vitro
Juglans regia is one of the most important nut crops rich in oil, protein and vitamins, and its high value timber is used for furniture, veneer and gunstock production. However, the propagation of J. regia is strongly dependent on the genotype. Slow in vitro growth results in low propagation rates a...
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Acta Hortic
2022
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oai:scholar.dlu.edu.vn:123456789-1161 |
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Thư viện Trường Đại học Đà Lạt |
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English |
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DKW medium indole‐3‐butyric acid root induction root expression |
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DKW medium indole‐3‐butyric acid root induction root expression Phạm, Ngọc Tuân A. Meier-Dinkel A. M. Ho¨ltken I. Wenzlitschke T. Winkelmann Factors affecting shoot multiplication and rooting of walnut (Juglans regia L.) in vitro |
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Juglans regia is one of the most important nut crops rich in oil, protein and vitamins, and its high value timber is used for furniture, veneer and gunstock production. However, the propagation of J. regia is strongly dependent on the genotype. Slow in vitro growth results in low propagation rates and difficulties are encountered using conventional rooting methods. Embryo axes dissected from mature nuts were used as primary explants for establishing shoot cultures. The growth of walnut shoot cultures was compared on media differing in nutrient
formulation (Rugini olive medium and DKW medium), concentrations of 6-benzyladenine (BA) (2.2-8.8 µM), solid and liquid medium and indole-3-butyric acid (IBA) concentration (4-20 µM). During the 21 days culture passage the Rugini medium gave significantly higher fresh mass and numbers of axillary shoots than DKW medium. The best BA concentration for J. regia explants was 2.2 µM BA. The results also indicated that solid medium (7 g L-1 agar) was better than liquid medium for shoot proliferation. The optimal IBA concentration in the induction medium for rooting of microcuttings was 12 µM IBA applied for 5 days in darkness before transfer
to root expression media. There was no significant difference among two root expression media (¼ DKW + Vermiculite and ¼ MS + Vermiculite) in rooting of microcuttings after 21 days. The acclimatization of rooted microcuttings was difficult because the tendency to dessicate of plantlets and because of the occurrence of
diseases due to high humidity in the greenhouse. Nevertheless, 80% of the J. regia plantlets were successfully established in the greenhouse. |
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Conference paper |
| author |
Phạm, Ngọc Tuân A. Meier-Dinkel A. M. Ho¨ltken I. Wenzlitschke T. Winkelmann |
| author_facet |
Phạm, Ngọc Tuân A. Meier-Dinkel A. M. Ho¨ltken I. Wenzlitschke T. Winkelmann |
| author_sort |
Phạm, Ngọc Tuân |
| title |
Factors affecting shoot multiplication and rooting of walnut (Juglans regia L.) in vitro |
| title_short |
Factors affecting shoot multiplication and rooting of walnut (Juglans regia L.) in vitro |
| title_full |
Factors affecting shoot multiplication and rooting of walnut (Juglans regia L.) in vitro |
| title_fullStr |
Factors affecting shoot multiplication and rooting of walnut (Juglans regia L.) in vitro |
| title_full_unstemmed |
Factors affecting shoot multiplication and rooting of walnut (Juglans regia L.) in vitro |
| title_sort |
factors affecting shoot multiplication and rooting of walnut (juglans regia l.) in vitro |
| publisher |
Acta Hortic |
| publishDate |
2022 |
| url |
http://scholar.dlu.edu.vn/handle/123456789/1161 |
| _version_ |
1768305951976194048 |
| spelling |
oai:scholar.dlu.edu.vn:123456789-11612022-10-03T09:00:53Z Factors affecting shoot multiplication and rooting of walnut (Juglans regia L.) in vitro Phạm, Ngọc Tuân A. Meier-Dinkel A. M. Ho¨ltken I. Wenzlitschke T. Winkelmann DKW medium indole‐3‐butyric acid root induction root expression Juglans regia is one of the most important nut crops rich in oil, protein and vitamins, and its high value timber is used for furniture, veneer and gunstock production. However, the propagation of J. regia is strongly dependent on the genotype. Slow in vitro growth results in low propagation rates and difficulties are encountered using conventional rooting methods. Embryo axes dissected from mature nuts were used as primary explants for establishing shoot cultures. The growth of walnut shoot cultures was compared on media differing in nutrient formulation (Rugini olive medium and DKW medium), concentrations of 6-benzyladenine (BA) (2.2-8.8 µM), solid and liquid medium and indole-3-butyric acid (IBA) concentration (4-20 µM). During the 21 days culture passage the Rugini medium gave significantly higher fresh mass and numbers of axillary shoots than DKW medium. The best BA concentration for J. regia explants was 2.2 µM BA. The results also indicated that solid medium (7 g L-1 agar) was better than liquid medium for shoot proliferation. The optimal IBA concentration in the induction medium for rooting of microcuttings was 12 µM IBA applied for 5 days in darkness before transfer to root expression media. There was no significant difference among two root expression media (¼ DKW + Vermiculite and ¼ MS + Vermiculite) in rooting of microcuttings after 21 days. The acclimatization of rooted microcuttings was difficult because the tendency to dessicate of plantlets and because of the occurrence of diseases due to high humidity in the greenhouse. Nevertheless, 80% of the J. regia plantlets were successfully established in the greenhouse. 1155 525-530 2022-10-02T23:48:38Z 2022-10-02T23:48:38Z 2017 Conference paper Bài báo đăng trên KYHT quốc tế (có ISBN) http://scholar.dlu.edu.vn/handle/123456789/1161 10.17660/ActaHortic.2017.1155.77 en Acta horticulturae 6th International ISHS Symposium on Production and Establishment of Micropropagated Plants, Sanremo 0567-7572 Cornu, D., and Jay‐Allemand, C. (1989). Micropropagation of hybrid walnut trees (Juglans nigra x Juglans regia) through culture and multiplication of embryos. Ann. Sci. For. 46 (Supplement), 113–116 http://dx.doi.org/ 10.1051/forest:19890523. Debergh, P., Harbaoui, Y., and Lemeur, R. (1981). Mass propagation of globe artichoke (Cynara scolymus): evaluation of different hypotheses to overcome vitrification with special reference to water potential. Physiol. Plant. 53 (2), 181–187 http://dx.doi.org/10.1111/j.1399‐3054.1981.tb04130.x. Driver, J.A., and Kuniyuki, A.H. (1984). In vitro propagation of paradox walnut rootstocks. Hortic. Sci. 19, 506– 509. Jay‐Allemand, C., and Cornu, D. (1986). Culture in vitro d’embryons isolés de noyer commun (Juglans regia L.). Ann. Sci. For. 43 (2), 189–198 http://dx.doi.org/10.1051/forest:19860205. Leslie, C., and McGranahan, G. (1992). Micropropagation of Persian walnut (Juglans regia L.). In High‐Tech and Micropropagation II, Y.P.S. Bajaj, ed. (Springer Berlin Heidelberg), p.136–150 http://dx.doi.org/10.1007/978‐3‐ 642‐76422‐6_7. Murashige, T., and Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant. 15 (3), 473–497 http://dx.doi.org/10.1111/j.1399‐3054.1962.tb08052.x. Payghamzadeh, K., and Kazemitabar, S.K. (2010). The effects of BAP, IBA and genotypes on in vitro germination of immature walnut embryos. Int. J. Plant Prod. 4, 309–322. Rugini, E. (1984). In vitro propagation of some olive (Olea europaea sativa L.) cultivars with different root‐ability, and medium development using analytical data from developing shoots and embryos. Sci. Hortic. (Amsterdam) 24 (2), 123–134 http://dx.doi.org/10.1016/0304‐4238(84)90143‐2. Šedivá, J., Vlašínová, H., and Mertelík, J. (2013). Shoot regeneration from various explants of horse chestnut (Aesculus hippocastanum L.).Sci. Hortic (Amsterdam)161,223–227http://dx.doi.org/10.1016/j.scienta.2013.06.030. Toosi, S., Dilmagani, K., and Hikmatshoar, H. (2010). Proliferation of Juglans regia L. by in vitro Embryo culture. Journal of Cell Biology and Genetics 1, 12–19. Acta Hortic Proc. VI Int. Symp. on Production and Establishment of Micropropagated Plants |