Comparative transcriptome and metabolome analyses of four Panax species explore the dynamics of metabolite biosynthesis
Background The genus Panax in the Araliaceae family has been used as traditional medicinal plants worldwide and is known to biosynthesize ginsenosides and phytosterols. However, genetic variation between Panax species has influenced their biosynthetic pathways is not fully understood. Methods Sim...
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2022
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oai:scholar.dlu.edu.vn:123456789-13072024-04-07T22:52:03Z Comparative transcriptome and metabolome analyses of four Panax species explore the dynamics of metabolite biosynthesis Hyunjin Koo Yun Sun Lee Nguyễn, Văn Bình Vo Ngoc Linh Giang Hyun Jo Koo Hyun-Seung Park Padmanaban Mohanan Young Hun Song Byeol Ryu Kyo Bin Kang Sang Hyun Sung Tae-Jin Yang Adventitious root, assembly, Oxidosqualene cyclase, species, Specialized metabolite profiling Background The genus Panax in the Araliaceae family has been used as traditional medicinal plants worldwide and is known to biosynthesize ginsenosides and phytosterols. However, genetic variation between Panax species has influenced their biosynthetic pathways is not fully understood. Methods Simultaneous analysis of transcriptomes and metabolomes obtained from adventitious roots of two tetraploid species (Panax ginseng and P. quinquefolius) and two diploid species (P. notoginseng and P. vietnamensis) revealed the diversity of their metabolites and related gene expression profiles. Results The transcriptome analysis showed that 2,3-OXIDOSQUALENE CYCLASEs (OSCs) involved in phytosterol biosynthesis are upregulated in the diploid species, while the expression of OSCs contributing to ginsenoside biosynthesis is higher in the tetraploid species. In agreement with these results, the contents of dammarenediol-type ginsenosides were higher in the tetraploid species relative to the diploid species. Conclusion These results suggest that a whole-genome duplication event has influenced the triterpene biosynthesis pathway in tetraploid Panax species during their evolution or ecological adaptation. This study provides a basis for further efforts to explore the genetic variation of the Panax genus. 2022-10-12T08:26:06Z 2022-10-12T08:26:06Z 2022 Journal article Bài báo đăng trên tạp chí thuộc ISI, bao gồm book chapter 1226-8453 https://scholar.dlu.edu.vn/handle/123456789/1307 10.1016/j.jgr.2022.07.001 https://www.sciencedirect.com/science/article/pii/S1226845322000884 en Journal of Ginseng Research |
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Thư viện Trường Đại học Đà Lạt |
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Thư viện số |
| language |
English |
| topic |
Adventitious root, assembly, Oxidosqualene cyclase, species, Specialized metabolite profiling |
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Adventitious root, assembly, Oxidosqualene cyclase, species, Specialized metabolite profiling Hyunjin Koo Yun Sun Lee Nguyễn, Văn Bình Vo Ngoc Linh Giang Hyun Jo Koo Hyun-Seung Park Padmanaban Mohanan Young Hun Song Byeol Ryu Kyo Bin Kang Sang Hyun Sung Tae-Jin Yang Comparative transcriptome and metabolome analyses of four Panax species explore the dynamics of metabolite biosynthesis |
| description |
Background
The genus Panax in the Araliaceae family has been used as traditional medicinal plants worldwide and is known to biosynthesize ginsenosides and phytosterols. However, genetic variation between Panax species has influenced their biosynthetic pathways is not fully understood.
Methods
Simultaneous analysis of transcriptomes and metabolomes obtained from adventitious roots of two tetraploid species (Panax ginseng and P. quinquefolius) and two diploid species (P. notoginseng and P. vietnamensis) revealed the diversity of their metabolites and related gene expression profiles.
Results
The transcriptome analysis showed that 2,3-OXIDOSQUALENE CYCLASEs (OSCs) involved in phytosterol biosynthesis are upregulated in the diploid species, while the expression of OSCs contributing to ginsenoside biosynthesis is higher in the tetraploid species. In agreement with these results, the contents of dammarenediol-type ginsenosides were higher in the tetraploid species relative to the diploid species.
Conclusion
These results suggest that a whole-genome duplication event has influenced the triterpene biosynthesis pathway in tetraploid Panax species during their evolution or ecological adaptation. This study provides a basis for further efforts to explore the genetic variation of the Panax genus. |
| format |
Journal article |
| author |
Hyunjin Koo Yun Sun Lee Nguyễn, Văn Bình Vo Ngoc Linh Giang Hyun Jo Koo Hyun-Seung Park Padmanaban Mohanan Young Hun Song Byeol Ryu Kyo Bin Kang Sang Hyun Sung Tae-Jin Yang |
| author_facet |
Hyunjin Koo Yun Sun Lee Nguyễn, Văn Bình Vo Ngoc Linh Giang Hyun Jo Koo Hyun-Seung Park Padmanaban Mohanan Young Hun Song Byeol Ryu Kyo Bin Kang Sang Hyun Sung Tae-Jin Yang |
| author_sort |
Hyunjin Koo |
| title |
Comparative transcriptome and metabolome analyses of four Panax species explore the dynamics of metabolite biosynthesis |
| title_short |
Comparative transcriptome and metabolome analyses of four Panax species explore the dynamics of metabolite biosynthesis |
| title_full |
Comparative transcriptome and metabolome analyses of four Panax species explore the dynamics of metabolite biosynthesis |
| title_fullStr |
Comparative transcriptome and metabolome analyses of four Panax species explore the dynamics of metabolite biosynthesis |
| title_full_unstemmed |
Comparative transcriptome and metabolome analyses of four Panax species explore the dynamics of metabolite biosynthesis |
| title_sort |
comparative transcriptome and metabolome analyses of four panax species explore the dynamics of metabolite biosynthesis |
| publishDate |
2022 |
| url |
https://scholar.dlu.edu.vn/handle/123456789/1307 https://www.sciencedirect.com/science/article/pii/S1226845322000884 |
| _version_ |
1798256926621958144 |