Identification of candidate UDP-glycosyltransferases involved in protopanaxadiol-type ginsenoside biosynthesis in Panax ginseng
Ginsenosides are dammarane-type or triterpenoidal saponins that contribute to the various pharmacological activities of the medicinal herb Panax ginseng. The putative biosynthetic pathway for ginsenoside biosynthesis is known in P. ginseng, as are some of the transcripts and enzyme-encoding genes. H...
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oai:scholar.dlu.edu.vn:123456789-13112022-10-12T08:32:59Z Identification of candidate UDP-glycosyltransferases involved in protopanaxadiol-type ginsenoside biosynthesis in Panax ginseng Kang, Kyo Bin Jayakodi, Murukarthick Lee, Yun Sun Nguyễn, Văn Bình Park, Hyun-Seung Koo, Hyun Jo Choi, Ik Young Kim, Dae Hyun Chung, You Jin Ryu, Byeol Lee, Dong Young Sung, Sang Hyun Yang, Tae-Jin Ginsenosides are dammarane-type or triterpenoidal saponins that contribute to the various pharmacological activities of the medicinal herb Panax ginseng. The putative biosynthetic pathway for ginsenoside biosynthesis is known in P. ginseng, as are some of the transcripts and enzyme-encoding genes. However, few genes related to the UDP-glycosyltransferases (UGTs), enzymes that mediate glycosylation processes in final saponin biosynthesis, have been identified. Here, we generated three replicated Illumina RNA-Seq datasets from the adventitious roots of P. ginseng cultivar Cheongsun (CS) after 0, 12, 24, and 48 h of treatment with methyl jasmonate (MeJA). Using the same CS cultivar, metabolomic data were also generated at 0 h and every 12-24 h thereafter until 120 h of MeJA treatment. Differential gene expression, phylogenetic analysis, and metabolic profiling were used to identify candidate UGTs. Eleven candidate UGTs likely to be involved in ginsenoside glycosylation were identified. Eight of these were considered novel UGTs, newly identified in this study, and three were matched to previously characterized UGTs in P. ginseng. Phylogenetic analysis further asserted their association with ginsenoside biosynthesis. Additionally, metabolomic analysis revealed that the newly identified UGTs might be involved in the elongation of glycosyl chains of ginsenosides, especially of protopanaxadiol (PPD)-type ginsenosides. 2022-10-12T08:32:53Z 2022-10-12T08:32:53Z 2018 Journal article Bài báo đăng trên tạp chí thuộc ISI, bao gồm book chapter http://scholar.dlu.edu.vn/handle/123456789/1311 10.1038/s41598-018-30262-7 30082711 en Scientific reports |
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Thư viện Trường Đại học Đà Lạt |
| collection |
Thư viện số |
| language |
English |
| description |
Ginsenosides are dammarane-type or triterpenoidal saponins that contribute to the various pharmacological activities of the medicinal herb Panax ginseng. The putative biosynthetic pathway for ginsenoside biosynthesis is known in P. ginseng, as are some of the transcripts and enzyme-encoding genes. However, few genes related to the UDP-glycosyltransferases (UGTs), enzymes that mediate glycosylation processes in final saponin biosynthesis, have been identified. Here, we generated three replicated Illumina RNA-Seq datasets from the adventitious roots of P. ginseng cultivar Cheongsun (CS) after 0, 12, 24, and 48 h of treatment with methyl jasmonate (MeJA). Using the same CS cultivar, metabolomic data were also generated at 0 h and every 12-24 h thereafter until 120 h of MeJA treatment. Differential gene expression, phylogenetic analysis, and metabolic profiling were used to identify candidate UGTs. Eleven candidate UGTs likely to be involved in ginsenoside glycosylation were identified. Eight of these were considered novel UGTs, newly identified in this study, and three were matched to previously characterized UGTs in P. ginseng. Phylogenetic analysis further asserted their association with ginsenoside biosynthesis. Additionally, metabolomic analysis revealed that the newly identified UGTs might be involved in the elongation of glycosyl chains of ginsenosides, especially of protopanaxadiol (PPD)-type ginsenosides. |
| format |
Journal article |
| author |
Kang, Kyo Bin Jayakodi, Murukarthick Lee, Yun Sun Nguyễn, Văn Bình Park, Hyun-Seung Koo, Hyun Jo Choi, Ik Young Kim, Dae Hyun Chung, You Jin Ryu, Byeol Lee, Dong Young Sung, Sang Hyun Yang, Tae-Jin |
| spellingShingle |
Kang, Kyo Bin Jayakodi, Murukarthick Lee, Yun Sun Nguyễn, Văn Bình Park, Hyun-Seung Koo, Hyun Jo Choi, Ik Young Kim, Dae Hyun Chung, You Jin Ryu, Byeol Lee, Dong Young Sung, Sang Hyun Yang, Tae-Jin Identification of candidate UDP-glycosyltransferases involved in protopanaxadiol-type ginsenoside biosynthesis in Panax ginseng |
| author_facet |
Kang, Kyo Bin Jayakodi, Murukarthick Lee, Yun Sun Nguyễn, Văn Bình Park, Hyun-Seung Koo, Hyun Jo Choi, Ik Young Kim, Dae Hyun Chung, You Jin Ryu, Byeol Lee, Dong Young Sung, Sang Hyun Yang, Tae-Jin |
| author_sort |
Kang, Kyo Bin |
| title |
Identification of candidate UDP-glycosyltransferases involved in protopanaxadiol-type ginsenoside biosynthesis in Panax ginseng |
| title_short |
Identification of candidate UDP-glycosyltransferases involved in protopanaxadiol-type ginsenoside biosynthesis in Panax ginseng |
| title_full |
Identification of candidate UDP-glycosyltransferases involved in protopanaxadiol-type ginsenoside biosynthesis in Panax ginseng |
| title_fullStr |
Identification of candidate UDP-glycosyltransferases involved in protopanaxadiol-type ginsenoside biosynthesis in Panax ginseng |
| title_full_unstemmed |
Identification of candidate UDP-glycosyltransferases involved in protopanaxadiol-type ginsenoside biosynthesis in Panax ginseng |
| title_sort |
identification of candidate udp-glycosyltransferases involved in protopanaxadiol-type ginsenoside biosynthesis in panax ginseng |
| publishDate |
2022 |
| url |
http://scholar.dlu.edu.vn/handle/123456789/1311 |
| _version_ |
1768306002489245696 |