Liposomal silibinin as a potential radioprotector of human lymphocytes in the treatment of non-small cell lung cancer
This study aimed to investigate the radioprotective effect of liposomal silibinin (Lip-SIL) on human lymphocytes in the treatment of non-small lung cancer cells using a combined method of cell viability assay and cytokinesis-block micronucleus assay for a better evaluation of whether one active c...
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| Định dạng: | Journal article |
| Ngôn ngữ: | English |
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2022
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| Truy cập trực tuyến: | http://scholar.dlu.edu.vn/handle/123456789/1574 |
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| Thư viện lưu trữ: | Thư viện Trường Đại học Đà Lạt |
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oai:scholar.dlu.edu.vn:123456789-1574 |
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| record_format |
dspace |
| institution |
Thư viện Trường Đại học Đà Lạt |
| collection |
Thư viện số |
| language |
English |
| topic |
Liposomes, lung cancer, radioprotection, radiotherapy, silibinin |
| spellingShingle |
Liposomes, lung cancer, radioprotection, radiotherapy, silibinin Nguyễn, Thị Huỳnh Nga Liposomal silibinin as a potential radioprotector of human lymphocytes in the treatment of non-small cell lung cancer |
| description |
This study aimed to investigate the radioprotective effect of liposomal
silibinin (Lip-SIL) on human lymphocytes in the treatment of non-small lung cancer
cells using a combined method of cell viability assay and cytokinesis-block
micronucleus assay for a better evaluation of whether one active compound is suitable
to be used as a radioprotector in radiotherapy or not. Materials and Methods: Firstly,
Lip-SIL was prepared by the lipid film hydration method combined with sonication.
Secondly, penetration of Lip-SIL into cells was observed by fluorescence microscopy.
Finally, the potential application of Lip-SIL as a radioprotector of human lymphocytes
in the treatment of non-small cell lung cancer was evaluated using the above
combined method with A549 cell line as a model. Results: The successfully prepared
Lip-SIL had a spherical shape and good physical characteristics (particle size of
approximately 83.9 nm, zeta potential of -20.6 mV, encapsulation efficiency of 28.8 %
and payload of 5.1 %). At a SIL concentration of 10 μg/mL, Lip-SIL exhibited the highest
radioprotection for lymphocytes, but showed no radioprotection or even increased
genotoxicity in human lung cancer A549 cells. Conclusion: Lip-SIL is a potential
protector of human lymphocytes during radiotherapy in the treatment of non-small
lung cancer. Moreover, the results of this study also imply that the radioprotection
ability of bioactive compounds for normal cells is not only based on their scavenging
activity on reactive oxygen species (ROS) but also on their mechanisms of intracellular
activations. |
| format |
Journal article |
| author |
Nguyễn, Thị Huỳnh Nga |
| author_facet |
Nguyễn, Thị Huỳnh Nga |
| author_sort |
Nguyễn, Thị Huỳnh Nga |
| title |
Liposomal silibinin as a potential radioprotector of human lymphocytes in the treatment of non-small cell lung cancer |
| title_short |
Liposomal silibinin as a potential radioprotector of human lymphocytes in the treatment of non-small cell lung cancer |
| title_full |
Liposomal silibinin as a potential radioprotector of human lymphocytes in the treatment of non-small cell lung cancer |
| title_fullStr |
Liposomal silibinin as a potential radioprotector of human lymphocytes in the treatment of non-small cell lung cancer |
| title_full_unstemmed |
Liposomal silibinin as a potential radioprotector of human lymphocytes in the treatment of non-small cell lung cancer |
| title_sort |
liposomal silibinin as a potential radioprotector of human lymphocytes in the treatment of non-small cell lung cancer |
| publishDate |
2022 |
| url |
http://scholar.dlu.edu.vn/handle/123456789/1574 |
| _version_ |
1768306090175365120 |
| spelling |
oai:scholar.dlu.edu.vn:123456789-15742022-11-09T06:40:11Z Liposomal silibinin as a potential radioprotector of human lymphocytes in the treatment of non-small cell lung cancer Nguyễn, Thị Huỳnh Nga Liposomes, lung cancer, radioprotection, radiotherapy, silibinin This study aimed to investigate the radioprotective effect of liposomal silibinin (Lip-SIL) on human lymphocytes in the treatment of non-small lung cancer cells using a combined method of cell viability assay and cytokinesis-block micronucleus assay for a better evaluation of whether one active compound is suitable to be used as a radioprotector in radiotherapy or not. Materials and Methods: Firstly, Lip-SIL was prepared by the lipid film hydration method combined with sonication. Secondly, penetration of Lip-SIL into cells was observed by fluorescence microscopy. Finally, the potential application of Lip-SIL as a radioprotector of human lymphocytes in the treatment of non-small cell lung cancer was evaluated using the above combined method with A549 cell line as a model. Results: The successfully prepared Lip-SIL had a spherical shape and good physical characteristics (particle size of approximately 83.9 nm, zeta potential of -20.6 mV, encapsulation efficiency of 28.8 % and payload of 5.1 %). At a SIL concentration of 10 μg/mL, Lip-SIL exhibited the highest radioprotection for lymphocytes, but showed no radioprotection or even increased genotoxicity in human lung cancer A549 cells. Conclusion: Lip-SIL is a potential protector of human lymphocytes during radiotherapy in the treatment of non-small lung cancer. Moreover, the results of this study also imply that the radioprotection ability of bioactive compounds for normal cells is not only based on their scavenging activity on reactive oxygen species (ROS) but also on their mechanisms of intracellular activations. 2022-11-09T03:40:46Z 2022-11-09T03:40:46Z 2022 Journal article Bài báo đăng trên tạp chí thuộc ISI, bao gồm book chapter Khoa học y, dược http://scholar.dlu.edu.vn/handle/123456789/1574 10.52547/ijrr.20.3.1 en 1. Siegel RL, Miller KD, Jemal A (2020) Cancer statistics, 2020. CA Cancer J Clin, 70: 7-30. 2. Azzam EI, Jay-Gerin JP, Pain D (2012) Ionizing radiation-induced metabolic oxidative stress and prolonged cell injury. Cancer Lett, 327: 48-60. 3. Abratt RP and Morgan GW (2002) Lung toxicity following chest irradiation in patients with lung cancer. Lung Cancer, 35: 103-109. 4. Kong FM, Hayman JA, Griffith KA, Kalemkerian GP, Arenberg D, Lyons S, Turrisi A, Lichter A, Fraass B, Eisbruch A, Lawrence TS, Haken RKT (2006) Final toxicity results of a radiation-dose escalation study in patients with non-small-cell lung cancer (NSCLC): predictors for radiation pneumonitis and fibrosis. Int J Radiat Oncol Biol Phys, 65: 1075-1086. 5. Nguyen MH, Duy PN, Dong B, Nguyen THN, Bui CB, Hadinoto K (2017) Radioprotective activity of curcumin-encapsulated liposomes against genotoxicity caused by gamma Cobalt-60 irradiation in human blood cells. Int J Radiat Biol, 93: 1267-1273. 6. Xie X, Gong S, Jin H, Yang P, Xu T, Cai Y, Guo C, Zhang R, Lou F, Yang W, Wang H (2020) Radiation-induced lymphopenia correlates with survival in nasopharyngeal carcinoma: impact of treatment modality and the baseline lymphocyte count. Radiat Oncol, 15: 65. 7. 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Wu JW, Lin LC, Hung SC, Chi CW, Tsai TH (2007) Analysis of silibinin in rat plasma and bile for hepatobiliary excretion and oral bioavailability application. J Pharm Biomed Anal, 45: 635-641. 17. Fatehi D, Mohammadi M, Shekarchi B, Shabani A, Seify M, Rostamzadeh A (2018) Radioprotective effects of Silymarin on the sperm parameters of NMRI mice irradiated with gamma-rays. J Photochem Photobiol B, 178: 489-495. |