Neuroprotection by NGF and BDNF against neurotoxin-exerted apoptotic death in neural stem cells are mediated through Trk receptors, activating PI3-kinase and MAPK pathways
Neural stem cells (NSC) undergo apoptotic cell death during development of nervous system and in adult. However, little is known about the biochemical regulation of neuroprotection by neurotrophin in these cells. In this report, we demonstrate that Staurosporine (STS) and Etoposide (ETS) induce...
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2022
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Thư viện Trường Đại học Đà Lạt |
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English |
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Neural stem cells, Staurosporine, Etoposide Apoptosis BDNF NGF |
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Neural stem cells, Staurosporine, Etoposide Apoptosis BDNF NGF Nguyễn, Thị Huỳnh Nga Neuroprotection by NGF and BDNF against neurotoxin-exerted apoptotic death in neural stem cells are mediated through Trk receptors, activating PI3-kinase and MAPK pathways |
| description |
Neural stem cells (NSC) undergo apoptotic cell
death during development of nervous system and in adult.
However, little is known about the biochemical regulation
of neuroprotection by neurotrophin in these cells. In this
report, we demonstrate that Staurosporine (STS) and Etoposide
(ETS) induced apoptotic cell death of NSC by a
mechanism requiring Caspase 3 activation, poly (ADPribose)
polymerase and Lamin A/C cleavage. Although
C17.2 cells revealed higher mRNA level of p75 neurotrophin
receptor (p75NTR) compared with TrkA or TrkB
receptor, neuroprotective effect of both nerve growth factor
(NGF) and brain-derived growth factor (BDNF) mediated
through the activation of tropomyosin receptor kinase (Trk)
receptors. Moreover, both NGF and BDNF induced the
activation of the phosphatidylinositide 3 kinase (PI3K)/Akt
and the mitogen-activated protein kinase (MAPK) pathway.
Inhibition of Trk receptor by K252a reduced PARP
cleavage as well as cell viability, whereas inhibition of
p75NTR did not affect the effect of neurotrophin on neurotoxic
insults. Thus our studies indicate that the protective
effect of NGF and BDNF in NSC against apoptotic stimuli
is mediated by the PI3K/Akt and MAPK signaling pathway
via Trk receptors. |
| format |
Journal article |
| author |
Nguyễn, Thị Huỳnh Nga |
| author_facet |
Nguyễn, Thị Huỳnh Nga |
| author_sort |
Nguyễn, Thị Huỳnh Nga |
| title |
Neuroprotection by NGF and BDNF against neurotoxin-exerted apoptotic death in neural stem cells are mediated through Trk receptors, activating PI3-kinase and MAPK pathways |
| title_short |
Neuroprotection by NGF and BDNF against neurotoxin-exerted apoptotic death in neural stem cells are mediated through Trk receptors, activating PI3-kinase and MAPK pathways |
| title_full |
Neuroprotection by NGF and BDNF against neurotoxin-exerted apoptotic death in neural stem cells are mediated through Trk receptors, activating PI3-kinase and MAPK pathways |
| title_fullStr |
Neuroprotection by NGF and BDNF against neurotoxin-exerted apoptotic death in neural stem cells are mediated through Trk receptors, activating PI3-kinase and MAPK pathways |
| title_full_unstemmed |
Neuroprotection by NGF and BDNF against neurotoxin-exerted apoptotic death in neural stem cells are mediated through Trk receptors, activating PI3-kinase and MAPK pathways |
| title_sort |
neuroprotection by ngf and bdnf against neurotoxin-exerted apoptotic death in neural stem cells are mediated through trk receptors, activating pi3-kinase and mapk pathways |
| publishDate |
2022 |
| url |
http://scholar.dlu.edu.vn/handle/123456789/1575 |
| _version_ |
1768306090536075264 |
| spelling |
oai:scholar.dlu.edu.vn:123456789-15752022-11-09T06:40:22Z Neuroprotection by NGF and BDNF against neurotoxin-exerted apoptotic death in neural stem cells are mediated through Trk receptors, activating PI3-kinase and MAPK pathways Nguyễn, Thị Huỳnh Nga Neural stem cells, Staurosporine, Etoposide Apoptosis BDNF NGF Neural stem cells (NSC) undergo apoptotic cell death during development of nervous system and in adult. However, little is known about the biochemical regulation of neuroprotection by neurotrophin in these cells. In this report, we demonstrate that Staurosporine (STS) and Etoposide (ETS) induced apoptotic cell death of NSC by a mechanism requiring Caspase 3 activation, poly (ADPribose) polymerase and Lamin A/C cleavage. Although C17.2 cells revealed higher mRNA level of p75 neurotrophin receptor (p75NTR) compared with TrkA or TrkB receptor, neuroprotective effect of both nerve growth factor (NGF) and brain-derived growth factor (BDNF) mediated through the activation of tropomyosin receptor kinase (Trk) receptors. Moreover, both NGF and BDNF induced the activation of the phosphatidylinositide 3 kinase (PI3K)/Akt and the mitogen-activated protein kinase (MAPK) pathway. Inhibition of Trk receptor by K252a reduced PARP cleavage as well as cell viability, whereas inhibition of p75NTR did not affect the effect of neurotrophin on neurotoxic insults. Thus our studies indicate that the protective effect of NGF and BDNF in NSC against apoptotic stimuli is mediated by the PI3K/Akt and MAPK signaling pathway via Trk receptors. 2022-11-09T04:34:46Z 2022-11-09T04:34:46Z 2009 Journal article Bài báo đăng trên tạp chí thuộc ISI, bao gồm book chapter Khoa học y, dược http://scholar.dlu.edu.vn/handle/123456789/1575 10.1007/s11064-008-9848-9 en 1. Kuan C-Y, Roth KA, Flavell RA et al (2000) Mechanisms of programmed cell death in the developing brain. Trends Neurosci 23:291–297. doi:10.1016/S0166-2236(00)01581-2 2. Snyder EY, Deitcher DL, Walsh C et al (1992) Multipotent neural cell lines can engraft and participate in development of mouse cerebellum. Cell 68:33–51. doi:10.1016/0092-8674(92) 90204-P 3. Snyder EY, Yoon C, Flax JD et al (1997) Multipotent neural precursors can differentiate toward replacement of neurons undergoing targeted apoptotic degeneration in adult mouse neocortex. Proc Natl Acad Sci USA 94:11663–11668. doi:10.1073/ pnas.94.21.11663 4. Homma S, Yaginuma H, Oppenheim RW (1994) Programmed cell death during the earliest stages of spinal cord development in the chick embryo: a possible means of early phenotypic selection. J Comp Neurol 345:377–395. doi:10.1002/cne.903450305 5. Slack RS, Skerjanc IS, Lach B et al (1995) Cells differentiating into neuroectoderm undergo apoptosis in the absence of functional retinoblastoma family proteins. J Cell Biol 129:779–788. doi:10.1083/jcb.129.3.779 6. Hagedorn L, Floris J, Suter U et al (2000) Autonomic neurogenesis and apoptosis are alternative fates of progenitor cell communities induced by TGFbeta. Dev Biol 228:57–72. doi: 10.1006/dbio.2000.9936 7. Levison SW, Rothstein RP, Brazel CY et al (2000) Selective apoptosis within the rat subependymal zone: a plausible mechanism for determining which lineages develop from neural stem cells. Dev Neurosci 22:106–115. doi:10.1159/000017432 8. Tamm C, Robertson JD, Sleeper E et al (2004) Differential regulation of the mitochondrial and death receptor pathways in neural stem cells. Eur J NeuroSci 19:2613–2621. doi:10.1111/ j.0953-816X.2004.03391.x 9. Cheng A, Chan SL, Milhavet O et al (2001) p38 MAP kinase mediates nitric oxide-induced apoptosis of neural progenitor cells. J Biol Chem 276:43320–43327. doi:10.1074/jbc.M10769 8200 10. Tamm C, Sabri F, Ceccatelli S (2008) Mitochondrial-mediated apoptosis in neural stem cells exposed to manganese. Toxicol Sci 101:310–320. doi:10.1093/toxsci/kfm267 11. Hennigan A, O’Callaghan RM, Kelly AM (2007) Neurotrophins and their receptors: roles in plasticity, neurodegeneration and neuroprotection. Biochem Soc Trans 35:424–427. doi:10.1042/ BST0350424 12. Rodriguez-Tebar A, Dechant G, Barde YA (1990) Binding of brain-derived neurotrophic factor to the nerve growth factor receptor. Neuron 4:487–492. doi:10.1016/0896-6273(90)90107-Q 13. Lindsay RM, Thoenen H, Barde YA (1985) Placode and neural crest-derived sensory neurons are responsive at early developmental stages to brain-derived neurotrophic factor. Dev Biol 112:319–328. doi:10.1016/0012-1606(85)90402-6 14. Sobue G, Yamamoto M, Doyu M et al (1998) Expression of mRNAs for neurotrophins (NGF, BDNF, and NT-3) and their receptors (p75NGFR, trk, trkB, and trkC) in human peripheral neuropathies. Neurochem Res 23:821–829. doi:10.1023/A:102 2434209787 15. Tucker KL, Meyer M, Barde YA (2001) Neurotrophins are required for nerve growth during development. Nat Neurosci 4:29–37. doi:10.1038/82868 16. Sofroniew MV, Howe CL, Mobley WC (2001) Nerve growth factor signaling, neuroprotection, and neural repair. Annu Rev Neurosci 24:1217–1281. doi:10.1146/annurev.neuro.24.1.1217 17. Huang EJ, Reichardt LF (2001) Neurotrophins: roles in neuronal development and function. Annu Rev Neurosci 24:677–736. doi: 10.1146/annurev.neuro.24.1.677 18. Huang EJ, Reichardt LF (2003) Trk receptors: roles in neuronal signal transduction. Annu Rev Biochem 72:609–642. doi: 10.1146/annurev.biochem.72.121801.161629 19. Snider WD (1994) Functions of the neurotrophins during nervous system development: what the knockouts are teaching us. Cell 77:627–638. doi:10.1016/0092-8674(94)90048-5 20. Meakin SO, Shooter EM (1992) The nerve growth factor family of receptors. Trends Neurosci 15:323–331. doi:10.1016/0166- 2236(92)90047-C 21. Carpenter CL, Auger KR, Chanudhuri M et al (1993) Phosphoinositide 3-kinase is activated by phosphopeptides that bind to the SH2 domains of the 85-kDa subunit. J Biol Chem 268:9478–9483 22. Sadowski HB, Shuai K, Darnell JE Jr et al (1993) A common nuclear signal transduction pathway activated by growth factor and cytokine receptors. Science 261:1739–1744. doi:10.1126/ science.8397445 23. Aronheim A, Engelberg D, Li N et al (1994) Membrane targeting of the nucleotide exchange factor Sos is sufficient for activating the Ras signaling pathway. Cell 78:949–961. doi:10.1016/0092- 8674(94)90271-2 24. Rodriguez-Viciana P, Warne PH, Dhand R et al (1994) Phosphatidylinositol- 3-OH kinase as a direct target of Ras. Nature 370:527–532 25. Cowley S, Paterson H, Kemp P et al (1994) Activation of MAP kinase kinase is necessary and sufficient for PC12 differentiation and for transformation of NIH 3T3 cells. Cell 77:841–852. doi: 10.1016/0092-8674(94)90133-3 26. Ye K, Hurt KJ, Wu FY et al (2000) Pike. A nuclear gtpase that enhances PI3kinase activity and is regulated by protein 4.1 N. Cell 103:919–930 27. Datta SR, Dudek H, Tao X et al (1997) Akt phosphorylation of BAD couples survival signals to the cell-intrinsic death machinery. Cell 91:231–241. doi:10.1016/S0092-8674(00)80405-5 28. del Peso L, Gonzalez-Garcia M, Page C et al (1997) Interleukin- 3-induced phosphorylation of BAD through the protein kinase Akt. Science 278:687–689 29. Cardone MH, Roy N, Stennicke HR et al (1998) Regulation of cell death protease caspase-9 by phosphorylation. Science 282:1318–1321. doi:10.1126/science.282.5392.1318 30. Biggs WHIII, Meisenhelder J, Hunter T et al (1999) Protein kinase B/Akt-mediated phosphorylation promotes nuclear exclusion of the winged helix transcription factor FKHR1. Proc Natl Acad Sci USA 96:7421–7426. doi:10.1073/pnas.96.13. 7421 31. Brunet A, Bonni A, Zigmond MJ et al (1999) Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor. Cell 96:857–868. doi:10.1016/S0092-8674(00) 80595-4 32. Kops GJ, de Ruiter ND, De Vries-Smits AM et al (1999) Direct control of the Forkhead transcription factor AFX by protein kinase B. Nature 398:630–634 33. Miyashita T, Reed JC (1995) Tumor suppressor p53 is a direct transcriptional activator of the human bax gene. Cell 80:293–299. doi:10.1016/0092-8674(95)90513-8 34. Yamaguchi A, Tamatani M, Matsuzaki H et al (2001) Akt activation protects hippocampal neurons from apoptosis by inhibiting transcriptional activity of p53. J Biol Chem 276:5256–5264. doi: 10.1074/jbc.M008552200 35. Ahn JY, Liu X, Liu Z et al (2006) Nuclear Akt associates with PKC-phosphorylated Ebp1, preventing DNA fragmentation by inhibition of caspase-activated DNase. EMBO J 25:2083–2095. doi:10.1038/sj.emboj.7601111 36. Marshall CJ (1995) Specificity of receptor tyrosine kinase signaling: transient versus sustained extracellular signal-regulated kinase activation. Cell 80:179–185. doi:10.1016/0092-8674(95) 90401-8 37. Robinson MJ, Cobb MH (1997) Mitogen-activated protein kinase pathways. Curr Opin Cell Biol 9:180–186. doi:10.1016/S0955- 0674(97)80061-0 38. Butler AA, Yakar S, Gewolb IH et al (1998) Insulin-like growth factor-I receptor signal transduction: at the interface between physiology and cell biology. Comp Biochem Physiol B Biochem Mol Biol 121:19–26. doi:10.1016/S0305-0491(98)10106-2 39. Bonni A, Brunet A, West AE et al (1999) Cell survival promoted by the Ras-MAPK signaling pathway by transcription-dependent and -independent mechanisms. Science 286:1358–1362. doi: 10.1126/science.286.5443.1358 40. Niles LP, Armstrong KJ, Rincon Castro LM et al (2004) Neural stem cells express melatonin receptors and neurotrophic factors: colocalization of the MT1 receptor with neuronal and glial markers. BMC Neurosci 5:41. doi:10.1186/1471-2202-5-41 41. Lu P, Jones LL, Snyder EY et al (2003) Neural stem cells constitutively secrete neurotrophic factors and promote extensive host axonal growth after spinal cord injury. Exp Neurol 181:115– 129. doi:10.1016/S0014-4886(03)00037-2 42. Soppet D, Escandon E, Maragos J et al (1991) The neurotrophic factors brain-derived neurotrophic factor and neurotrophin-3 are ligands for the trkB tyrosine kinase receptor. Cell 65:895–903. doi:10.1016/0092-8674(91)90396-G 43. Bai O, Wei Z, Lu W et al (2002) Protective effects of atypical antipsychotic drugs on PC12 cells after serum withdrawal. J Neurosci Res 69:278–283. doi:10.1002/jnr.10290 44. Perez-Pinera P, Hernandez T, Garcia-Suarez O et al (2007) The Trk tyrosine kinase inhibitor K252a regulates growth of lung adenocarcinomas. Mol Cell Biochem 295:19–26. doi:10.1007/ s11010-006-9267-7 45. Young D, Lawlor PA, Leone P et al (1999) Environmental enrichment inhibits spontaneous apoptosis, prevents seizures and is neuroprotective. Nat Med 5:448–453. doi:10.1038/7449 46. Biebl M, Cooper CM, Winkler J et al (2000) Analysis of neurogenesis and programmed cell death reveals a self-renewing capacity in the adult rat brain. Neurosci Lett 291:17–20. doi: 10.1016/S0304-3940(00)01368-9 47. Lee SM, Tole S, Grove E et al (2000) A local Wnt-3a signal is required for development of the mammalian hippocampus. Development 127:457–467 48. Zheng WH, Kar S, Quirion R (2002) Insulin-like growth factor-1- induced phosphorylation of transcription factor FKHRL1 is mediated by phosphatidylinositol 3-kinase/Akt kinase and role of this pathway in insulin-like growth factor-1-induced survival of cultured hippocampal neurons. Mol Pharmacol 62:225–233. doi: 10.1124/mol.62.2.225 49. Yao R, Cooper GM (1995) Requirement for phosphatidylinositol- 3 kinase in the prevention of apoptosis by nerve growth factor. Science 267:2003–2006. doi:10.1126/science.7701324 50. Skaper SD, Floreani M, Negro A et al (1998) Neurotrophins rescue cerebellar granule neurons from oxidative stress-mediated apoptotic death: selective involvement of phosphatidylinositol 3- kinase and the mitogen-activated protein kinase pathway. J Neurochem 70:1859–1868 51. Hetman M, Kanning K, Cavanaugh JE et al (1999) Neuroprotection by brain-derived neurotrophic factor is mediated by extracellular signal-regulated kinase and phosphatidylinositol 3- kinase. J Biol Chem 274:22569–22580. doi:10.1074/jbc.274.32. 22569 52. Crowder RJ, Freeman RS (1998) Phosphatidylinositol 3-kinase and Akt protein kinase are necessary and sufficient for the survival of nerve growth factor-dependent sympathetic neurons. J Neurosci 18:2933–2943 53. Mazzoni IE, Said FA, Aloyz R et al (1999) Ras regulates sympathetic neuron survival by suppressing the p53-mediated cell death pathway. J Neurosci 19:9716–9727 54. Xue L, Murray JH, Tolkovsky AM (2000) The Ras/phosphatidylinositol 3-kinase and Ras/ERK pathways function as independent survival modules each of which inhibits a distinct apoptotic signaling pathway in sympathetic neurons. J Biol Chem 275:8817–8824. doi:10.1074/jbc.275.12.8817 55. Klesse LJ, Parada LF (1998) p21 Ras and phosphatidylinositol-3 kinase are required for survival of wild-type and NF1 mutant sensory neurons. J Neurosci 18:10420–10428 56. Dolcet X, Egea J, Soler RM et al (1999) Activation of phosphatidylinositol 3-kinase, but not extracellular-regulated kinases, is necessary to mediate brain-derived neurotrophic factor-induced motoneuron survival. J Neurochem 73:521–531. doi:10.1046/ j.1471-4159.1999.0730521.x 57. Almeida RD, Manadas BJ, Melo CV et al (2005) Neuroprotection by BDNF against glutamate-induced apoptotic cell death is mediated by ERK and PI3-kinase pathways. Cell Death Differ 12:1329–1343. doi:10.1038/sj.cdd.4401662 58. Chang SH, Poser S, Xia Z (2004) A novel role for serum response factor in neuronal survival. J Neurosci 24:2277–2285 |