XXIV World Allergy Congress 2015
Purpose: Pirfenidone has been shown to have anti-fibrotic and anti-inflammatory effects in the lungs. The purpose of this study was to evaluate the inhibitory effects of pirfenidone on transforming growth factor (TGF)-β1-induced myofibroblast differentiation and extracellular matrix accumulation. We...
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Springer
2023
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| Truy cập trực tuyến: | http://scholar.dlu.edu.vn/handle/123456789/1653 https://link.springer.com/article/10.1186/s40413-016-0096-1 |
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oai:scholar.dlu.edu.vn:123456789-16532023-01-04T09:59:53Z XXIV World Allergy Congress 2015 Trịnh, Thị Tú Anh Purpose: Pirfenidone has been shown to have anti-fibrotic and anti-inflammatory effects in the lungs. The purpose of this study was to evaluate the inhibitory effects of pirfenidone on transforming growth factor (TGF)-β1-induced myofibroblast differentiation and extracellular matrix accumulation. We also determined the molecular mechanisms of pirfenidone in nasal polyp-derived fibroblasts (NPDFs). Methods: NPDFs were isolated from nasal polyps from eight patients who had chronic rhinosinusitis with nasal polyp. Pirfenidone was used to treat TGF-β1-induced NPDFs. Cytotoxicity was evaluated using a 3-(4, 5-dimethylthiazol-2yl)-2, 5-diphenyl-tetrazolium bromide assay. Fibroblast migration was evaluated with scratch assays. Expression levels of α-smooth muscle actin (SMA), fibronectin, and phosphorylated Smad2/3 were determined by western blot and/or reverse transcription-polymerase chain reaction 9 1 1-206 2023-01-03T02:12:14Z 2023-01-03T02:12:14Z 2015 Conference object Bài báo đăng trên KYHT quốc tế (có ISBN) http://scholar.dlu.edu.vn/handle/123456789/1653 https://link.springer.com/article/10.1186/s40413-016-0096-1 vi Springer BioMed Central |
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Thư viện Trường Đại học Đà Lạt |
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Thư viện số |
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Vietnamese |
| description |
Purpose: Pirfenidone has been shown to have anti-fibrotic and anti-inflammatory effects in the lungs. The purpose of this study was to evaluate the inhibitory effects of pirfenidone on transforming growth factor (TGF)-β1-induced myofibroblast differentiation and extracellular matrix accumulation. We also determined the molecular mechanisms of pirfenidone in nasal polyp-derived fibroblasts (NPDFs).
Methods: NPDFs were isolated from nasal polyps from eight patients who had chronic rhinosinusitis with nasal polyp. Pirfenidone was used to treat TGF-β1-induced NPDFs. Cytotoxicity was evaluated using a 3-(4, 5-dimethylthiazol-2yl)-2, 5-diphenyl-tetrazolium bromide assay. Fibroblast migration was evaluated with scratch assays. Expression levels of α-smooth muscle actin (SMA), fibronectin, and phosphorylated Smad2/3 were determined by western blot and/or reverse transcription-polymerase chain reaction |
| format |
Conference object |
| author |
Trịnh, Thị Tú Anh |
| spellingShingle |
Trịnh, Thị Tú Anh XXIV World Allergy Congress 2015 |
| author_facet |
Trịnh, Thị Tú Anh |
| author_sort |
Trịnh, Thị Tú Anh |
| title |
XXIV World Allergy Congress 2015 |
| title_short |
XXIV World Allergy Congress 2015 |
| title_full |
XXIV World Allergy Congress 2015 |
| title_fullStr |
XXIV World Allergy Congress 2015 |
| title_full_unstemmed |
XXIV World Allergy Congress 2015 |
| title_sort |
xxiv world allergy congress 2015 |
| publisher |
Springer |
| publishDate |
2023 |
| url |
http://scholar.dlu.edu.vn/handle/123456789/1653 https://link.springer.com/article/10.1186/s40413-016-0096-1 |
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1768306225443766272 |