Phenology-regulated defence mechanisms as drivers for Fusarium basal rot in onion (Allium cepa)

Phenology-regulated defence mechanisms as drivers for Fusarium basal rot in onion (Allium cepa)

Fusarium basal rot (FBR), caused by Fusarium spp., is a serious impediment for onion production. In the present study, the interaction of onion (Allium cepa) var. Takstar F1 with Allium-derived isolates of the toxigenic species F. oxysporum and F. proliferatum was investigated at seedling and bulb s...

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Tác giả chính: Lê, Dũng
Định dạng: Journal article
Ngôn ngữ:English
Được phát hành: Wiley 2023
Những chủ đề:
Fusarium basal rot
RT-PCR
phenology
defense mechanism
Mycotoxin
phenylalanine ammonia-lyase
lipoxygenase
pectin methylesterases
chalcone
anthocyanidin
Truy cập trực tuyến:https://scholar.dlu.edu.vn/handle/123456789/2510
https://doi.org/10.1111/ppa.13574
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Thư viện lưu trữ: Thư viện Trường Đại học Đà Lạt
id oai:scholar.dlu.edu.vn:123456789-2510
record_format dspace
institution Thư viện Trường Đại học Đà Lạt
collection Thư viện số
language English
topic Fusarium basal rot
RT-PCR
phenology
defense mechanism
Mycotoxin
phenylalanine ammonia-lyase
lipoxygenase
pectin methylesterases
chalcone
anthocyanidin
spellingShingle Fusarium basal rot
RT-PCR
phenology
defense mechanism
Mycotoxin
phenylalanine ammonia-lyase
lipoxygenase
pectin methylesterases
chalcone
anthocyanidin
Lê, Dũng
Phenology-regulated defence mechanisms as drivers for Fusarium basal rot in onion (Allium cepa)
description Fusarium basal rot (FBR), caused by Fusarium spp., is a serious impediment for onion production. In the present study, the interaction of onion (Allium cepa) var. Takstar F1 with Allium-derived isolates of the toxigenic species F. oxysporum and F. proliferatum was investigated at seedling and bulb stage. Using a set of isolates with distinct virulence profiles, we provide evidence that the outcome of FBR is driven by the phenology of onion. Reverse transcription-quantitative PCR analysis revealed distinct expression of reliable marker genes for phenylalanine ammonia-lyase (PAL1, PAL2), lipoxygenase (LOX2), chalcone synthase (CHS), anthocyanidin synthase (ANS) and pectin methylesterases (PME) during the infection of seedlings and bulbs. These defence-related genes in seedlings were differentially expressed depending on the aggressiveness of Fusarium isolates. Despite these differential kinetics, the activated plant defence did not seem to be effective in protecting the seedlings from Fusarium infection and proliferation. Meanwhile, gene expression in the bulb organs correlated positively with FBR severity, indicating an ineffective defence response. Among the major metabolites of these Fusarium species, beauvericin was not toxic to either the seedlings or the bulbs, while fumonisin B1 appeared to be a virulence factor specific for the seedling stage. This points to differential roles of fumonisin B1 during an infection of F. proliferatum depending on the infected organ and the phenological stage of the onion host. We hypothesize that this variation, along with the differences within and between Fusarium species in terms of virulence and metabolism, contributes to the determination of disease outcome.
format Journal article
author Lê, Dũng
author_facet Lê, Dũng
author_sort Lê, Dũng
title Phenology-regulated defence mechanisms as drivers for Fusarium basal rot in onion (Allium cepa)
title_short Phenology-regulated defence mechanisms as drivers for Fusarium basal rot in onion (Allium cepa)
title_full Phenology-regulated defence mechanisms as drivers for Fusarium basal rot in onion (Allium cepa)
title_fullStr Phenology-regulated defence mechanisms as drivers for Fusarium basal rot in onion (Allium cepa)
title_full_unstemmed Phenology-regulated defence mechanisms as drivers for Fusarium basal rot in onion (Allium cepa)
title_sort phenology-regulated defence mechanisms as drivers for fusarium basal rot in onion (allium cepa)
publisher Wiley
publishDate 2023
url https://scholar.dlu.edu.vn/handle/123456789/2510
https://doi.org/10.1111/ppa.13574
_version_ 1785973145439567872
spelling oai:scholar.dlu.edu.vn:123456789-25102023-12-13T03:44:41Z Phenology-regulated defence mechanisms as drivers for Fusarium basal rot in onion (Allium cepa) Lê, Dũng Fusarium basal rot RT-PCR phenology defense mechanism Mycotoxin phenylalanine ammonia-lyase lipoxygenase pectin methylesterases chalcone anthocyanidin Fusarium basal rot (FBR), caused by Fusarium spp., is a serious impediment for onion production. In the present study, the interaction of onion (Allium cepa) var. Takstar F1 with Allium-derived isolates of the toxigenic species F. oxysporum and F. proliferatum was investigated at seedling and bulb stage. Using a set of isolates with distinct virulence profiles, we provide evidence that the outcome of FBR is driven by the phenology of onion. Reverse transcription-quantitative PCR analysis revealed distinct expression of reliable marker genes for phenylalanine ammonia-lyase (PAL1, PAL2), lipoxygenase (LOX2), chalcone synthase (CHS), anthocyanidin synthase (ANS) and pectin methylesterases (PME) during the infection of seedlings and bulbs. These defence-related genes in seedlings were differentially expressed depending on the aggressiveness of Fusarium isolates. Despite these differential kinetics, the activated plant defence did not seem to be effective in protecting the seedlings from Fusarium infection and proliferation. Meanwhile, gene expression in the bulb organs correlated positively with FBR severity, indicating an ineffective defence response. Among the major metabolites of these Fusarium species, beauvericin was not toxic to either the seedlings or the bulbs, while fumonisin B1 appeared to be a virulence factor specific for the seedling stage. This points to differential roles of fumonisin B1 during an infection of F. proliferatum depending on the infected organ and the phenological stage of the onion host. We hypothesize that this variation, along with the differences within and between Fusarium species in terms of virulence and metabolism, contributes to the determination of disease outcome. 71 1440– 1453 2023-06-10T02:09:34Z 2023-06-10T02:09:34Z 2022-04-27 Journal article Bài báo đăng trên tạp chí thuộc ISI, bao gồm book chapter https://scholar.dlu.edu.vn/handle/123456789/2510 https://doi.org/10.1111/ppa.13574 en Plant Pathology An, J.P., Liu, Y.J., Zhang, X.W., Bi, S.Q., Wang, X.F., You, C.X. et al. (2020) Dynamic regulation of anthocyanin biosynthesis at different light intensities by the BT2-TCP46-MYB1 module in apple. Journal of Experimental Botany, 71, 3094– 3109. 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