Chromosome-scale genome assembly for the duckweed Spirodela intermedia, integrating cytogenetic maps, PacBio and Oxford Nanopore libraries
monocot order Alismatales. They display the most rapid growth among flowering plants, vary ~ 14-fold in genome size and comprise five genera. Spirodela is the phylogenetically oldest genus with only two mainly asexually propagating species: S. polyrhiza (2n = 40; 160 Mbp/1C) and S. intermedia (2n...
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| Những tác giả chính: | , , , , , , , , , |
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| Formato: | Journal article |
| Idioma: | English |
| Publicado em: |
Nature Publishing Group UK
2023
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| Acesso em linha: | https://scholar.dlu.edu.vn/handle/123456789/3092 https://doi.org/10.1038/s41598-020-75728-9 |
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| Thư viện lưu trữ: | Thư viện Trường Đại học Đà Lạt |
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| Resumo: | monocot order Alismatales. They display the most rapid growth among flowering plants, vary ~ 14-fold
in genome size and comprise five genera. Spirodela is the phylogenetically oldest genus with only two
mainly asexually propagating species: S. polyrhiza (2n = 40; 160 Mbp/1C) and S. intermedia (2n = 36;
160 Mbp/1C). This study combined comparative cytogenetics and de novo genome assembly based
on PacBio, Illumina and Oxford Nanopore (ON) reads to obtain the first genome reference for S.
intermedia and to compare its genomic features with those of the sister species S. polyrhiza. Both
species’ genomes revealed little more than 20,000 putative protein-coding genes, very low rDNA copy
numbers and a low amount of repetitive sequences, mainly Ty3/gypsy retroelements. The detection of
a few new small chromosome rearrangements between both Spirodela species refined the karyotype
and the chromosomal sequence assignment for S. intermedia. |
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