Micropropagation of Lang Bian ginseng: an endemic medicinal plant
Micropropagation of Lang Bian ginseng (Panax vietnamensis var. langbianensis), an endemic medicinal plant, via somatic embryogenesis (SE) and rhizome formation were investigated. Rhizome explants disinfected with 0.15% AgNPs for 30 min resulted in the most effective surface disinfection (contamina...
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| Ngôn ngữ: | English |
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Springer Netherlands
2023
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| Truy cập trực tuyến: | https://scholar.dlu.edu.vn/handle/123456789/3172 |
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Thư viện Trường Đại học Đà Lạt |
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English |
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Micropropagation of Lang Bian ginseng (Panax vietnamensis var. langbianensis), an endemic medicinal plant, via somatic
embryogenesis (SE) and rhizome formation were investigated. Rhizome explants disinfected with 0.15% AgNPs for 30 min
resulted in the most effective surface disinfection (contamination, necrosis and survival with callus induction of 36.00%,
14.70% and 49.30%, respectively). Moreover, shoot regeneration (45.33%) and the number of shoots (5.2 shoots) from
0.15% AgNPs disinfected- rhizome were significantly higher than those in others after 12 weeks of culture. The leaf was cut
transverse thin cell layer (L-tTCL: 1 mm × 5 mm) and petiole cut longitudinally TCL (P-lTCL: 0.5 mm × 10 mm). L-tTCL
explant cultured on MS medium supplemented with 7 mg/L NAA gave 100% SE and 32.80 embryos; meanwhile, P-lTCL
explants also obtained 100% SE and 51.80 embryos in 1 mg/L 2,4-D treatment. Adventitious root formation and callus
induction were also observed in the auxin-supplemented treatments. In addition, 12-week-old secondary somatic embryos,
mainly cotyledonary stage, were obtained from the primary somatic embryo cultured on 1/2 MS medium supplemented with
1 mg/L 2,4-D. Plantlets derived from secondary embryos grew well with 63.34% rhizome formation, 1.27 cm in rhizome
length and 0.65 cm in rhizome diameter. Meanwhile, plantlets derived from adventitious shoots only induced adventitious
roots and callogenesis after 20 weeks of culture. The analysis of saponins by HPLC method showed that twenty-week-old
in vitro rhizomes had Rg1, Rd and Rb1. |
| format |
Journal article |
| author |
Truong, Thi Lan Anh Doan, Manh Dung Hoang, Thanh Tung Hoang, Dac Khai Nguyen, Thi Nhu Mai Vu, Quoc Luan Do, Manh Cuong Hoàng, Thị Như Phương Le, Thi Diem Nguyen, Quang Vinh Bui, Van The Vinh Nguyen, Phuong Thao Duong, Tan Nhut |
| spellingShingle |
Truong, Thi Lan Anh Doan, Manh Dung Hoang, Thanh Tung Hoang, Dac Khai Nguyen, Thi Nhu Mai Vu, Quoc Luan Do, Manh Cuong Hoàng, Thị Như Phương Le, Thi Diem Nguyen, Quang Vinh Bui, Van The Vinh Nguyen, Phuong Thao Duong, Tan Nhut Micropropagation of Lang Bian ginseng: an endemic medicinal plant |
| author_facet |
Truong, Thi Lan Anh Doan, Manh Dung Hoang, Thanh Tung Hoang, Dac Khai Nguyen, Thi Nhu Mai Vu, Quoc Luan Do, Manh Cuong Hoàng, Thị Như Phương Le, Thi Diem Nguyen, Quang Vinh Bui, Van The Vinh Nguyen, Phuong Thao Duong, Tan Nhut |
| author_sort |
Truong, Thi Lan Anh |
| title |
Micropropagation of Lang Bian ginseng: an endemic medicinal plant |
| title_short |
Micropropagation of Lang Bian ginseng: an endemic medicinal plant |
| title_full |
Micropropagation of Lang Bian ginseng: an endemic medicinal plant |
| title_fullStr |
Micropropagation of Lang Bian ginseng: an endemic medicinal plant |
| title_full_unstemmed |
Micropropagation of Lang Bian ginseng: an endemic medicinal plant |
| title_sort |
micropropagation of lang bian ginseng: an endemic medicinal plant |
| publisher |
Springer Netherlands |
| publishDate |
2023 |
| url |
https://scholar.dlu.edu.vn/handle/123456789/3172 |
| _version_ |
1783866428723560448 |
| spelling |
oai:scholar.dlu.edu.vn:123456789-31722023-11-15T08:47:01Z Micropropagation of Lang Bian ginseng: an endemic medicinal plant Truong, Thi Lan Anh Doan, Manh Dung Hoang, Thanh Tung Hoang, Dac Khai Nguyen, Thi Nhu Mai Vu, Quoc Luan Do, Manh Cuong Hoàng, Thị Như Phương Le, Thi Diem Nguyen, Quang Vinh Bui, Van The Vinh Nguyen, Phuong Thao Duong, Tan Nhut Micropropagation of Lang Bian ginseng (Panax vietnamensis var. langbianensis), an endemic medicinal plant, via somatic embryogenesis (SE) and rhizome formation were investigated. Rhizome explants disinfected with 0.15% AgNPs for 30 min resulted in the most effective surface disinfection (contamination, necrosis and survival with callus induction of 36.00%, 14.70% and 49.30%, respectively). Moreover, shoot regeneration (45.33%) and the number of shoots (5.2 shoots) from 0.15% AgNPs disinfected- rhizome were significantly higher than those in others after 12 weeks of culture. The leaf was cut transverse thin cell layer (L-tTCL: 1 mm × 5 mm) and petiole cut longitudinally TCL (P-lTCL: 0.5 mm × 10 mm). L-tTCL explant cultured on MS medium supplemented with 7 mg/L NAA gave 100% SE and 32.80 embryos; meanwhile, P-lTCL explants also obtained 100% SE and 51.80 embryos in 1 mg/L 2,4-D treatment. Adventitious root formation and callus induction were also observed in the auxin-supplemented treatments. In addition, 12-week-old secondary somatic embryos, mainly cotyledonary stage, were obtained from the primary somatic embryo cultured on 1/2 MS medium supplemented with 1 mg/L 2,4-D. Plantlets derived from secondary embryos grew well with 63.34% rhizome formation, 1.27 cm in rhizome length and 0.65 cm in rhizome diameter. Meanwhile, plantlets derived from adventitious shoots only induced adventitious roots and callogenesis after 20 weeks of culture. The analysis of saponins by HPLC method showed that twenty-week-old in vitro rhizomes had Rg1, Rd and Rb1. 151 3 565-578 2023-11-15T08:46:55Z 2023-11-15T08:46:55Z 2022-12 Journal article Bài báo đăng trên tạp chí thuộc ISI, bao gồm book chapter https://scholar.dlu.edu.vn/handle/123456789/3172 10.1007/s11240-022-02372-8 en Plant Cell, Tissue and Organ Culture (PCTOC) 1573-5044 Abdolinejad R, Shekafandeh A, Jowkar A, Gharaghani A, Alemzadeh A (2020) Indirect regeneration of Ficus carica by the TCL technique and genetic fidelity evaluation of the regenerated plants using flow cytometry and ISSR. Plant Cell Tissue Organ Cult 143(1):131–144. https:// doi. org/ 10. 1007/ s11240- 020- 01903-5 Ahn IO, Van Le B, Gendy C, Van Tran Than K (1996) Direct somatic embryogenesis through thin cell layer culture in Panax ginseng. 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