Tetraploid induction through somatic embryogenesis in Panax vietnamensis Ha et Grushv. by colchicine treatment
In the present study, the protocol of polyploid induction via somatic embryogenesis of the Ngoc Linh ginseng (Panax vietnamensis Ha et Grushv.) (2n = 2x = 24) was developed. Ex vitro leaf explants were disinfected with AgNPs at concentrations of 0.05, 0.1, 0.2 and 0.5 g/L for 5, 10, 15, 20 and 30...
Đã lưu trong:
| Những tác giả chính: | , , , , , , , , |
|---|---|
| Định dạng: | Journal article |
| Ngôn ngữ: | Vietnamese |
| Được phát hành: |
Elsevier
2023
|
| Truy cập trực tuyến: | https://scholar.dlu.edu.vn/handle/123456789/3173 |
| Các nhãn: |
Thêm thẻ
Không có thẻ, Là người đầu tiên thẻ bản ghi này!
|
| Thư viện lưu trữ: | Thư viện Trường Đại học Đà Lạt |
|---|
| id |
oai:scholar.dlu.edu.vn:123456789-3173 |
|---|---|
| record_format |
dspace |
| institution |
Thư viện Trường Đại học Đà Lạt |
| collection |
Thư viện số |
| language |
Vietnamese |
| description |
In the present study, the protocol of polyploid induction via somatic embryogenesis of the Ngoc Linh ginseng
(Panax vietnamensis Ha et Grushv.) (2n = 2x = 24) was developed. Ex vitro leaf explants were disinfected with
AgNPs at concentrations of 0.05, 0.1, 0.2 and 0.5 g/L for 5, 10, 15, 20 and 30 min and cultured on MS medium
supplemented with 1.0 mg/L 2,4-D, 0.5 mg/L NAA and 0.2 mg/L Kin for somatic embryogenesis. Globular
embryos induced from the leaf explants were treated with colchicine by immersed method at different concentrations
of 0, 0.1, 0.2, 0.3, 0.5 and 0.7% for 24, 36, 48 and 72 h. Then, these embryos were cultured on
medium supplemented with 1.0 mg/L 2,4-D, 0.5 mg/L NAA and 0.2 mg/L Kin for secondary embryos induction.
The ploidy level was identified by counting chromosomes of the root tip of plantlets derived from these secondary
embryos. The results showed that the survival rate and somatic embryo induction the highest (65.56%
and 29.89%, respectively) at 0.2 g/L AgNPs for 20 min. For colchicine treatment, the increase in the concentration
and exposure time of colchicine decreased the survival rate of explants and the number of secondary
embryos but increased the abnormal secondary embryo formation. Identifying the ploidy level showed that the
treatments with colchicine concentrations from 0.3% to 0.5% for 48 h lead to tetraploid induction rates from
22.22% to 25.92%. The results also revealed that the tetraploids (2n = 4x = 48) had larger stomatal size, lower
stomatal density, denser stomatal chloroplast density, and better growth than did the diploids. |
| format |
Journal article |
| author |
Truong, Hoai Phong Hoang, Thanh Tung Hoang, Dac Khai Nguyen, Thi Nhu Mai Vu, Quoc Luan Tran, Que Hoàng, Thị Như Phương Bui, Van The Vinh Duong, Tan Nhut |
| spellingShingle |
Truong, Hoai Phong Hoang, Thanh Tung Hoang, Dac Khai Nguyen, Thi Nhu Mai Vu, Quoc Luan Tran, Que Hoàng, Thị Như Phương Bui, Van The Vinh Duong, Tan Nhut Tetraploid induction through somatic embryogenesis in Panax vietnamensis Ha et Grushv. by colchicine treatment |
| author_facet |
Truong, Hoai Phong Hoang, Thanh Tung Hoang, Dac Khai Nguyen, Thi Nhu Mai Vu, Quoc Luan Tran, Que Hoàng, Thị Như Phương Bui, Van The Vinh Duong, Tan Nhut |
| author_sort |
Truong, Hoai Phong |
| title |
Tetraploid induction through somatic embryogenesis in Panax vietnamensis Ha et Grushv. by colchicine treatment |
| title_short |
Tetraploid induction through somatic embryogenesis in Panax vietnamensis Ha et Grushv. by colchicine treatment |
| title_full |
Tetraploid induction through somatic embryogenesis in Panax vietnamensis Ha et Grushv. by colchicine treatment |
| title_fullStr |
Tetraploid induction through somatic embryogenesis in Panax vietnamensis Ha et Grushv. by colchicine treatment |
| title_full_unstemmed |
Tetraploid induction through somatic embryogenesis in Panax vietnamensis Ha et Grushv. by colchicine treatment |
| title_sort |
tetraploid induction through somatic embryogenesis in panax vietnamensis ha et grushv. by colchicine treatment |
| publisher |
Elsevier |
| publishDate |
2023 |
| url |
https://scholar.dlu.edu.vn/handle/123456789/3173 |
| _version_ |
1783866429091610624 |
| spelling |
oai:scholar.dlu.edu.vn:123456789-31732023-11-15T08:53:14Z Tetraploid induction through somatic embryogenesis in Panax vietnamensis Ha et Grushv. by colchicine treatment Truong, Hoai Phong Hoang, Thanh Tung Hoang, Dac Khai Nguyen, Thi Nhu Mai Vu, Quoc Luan Tran, Que Hoàng, Thị Như Phương Bui, Van The Vinh Duong, Tan Nhut In the present study, the protocol of polyploid induction via somatic embryogenesis of the Ngoc Linh ginseng (Panax vietnamensis Ha et Grushv.) (2n = 2x = 24) was developed. Ex vitro leaf explants were disinfected with AgNPs at concentrations of 0.05, 0.1, 0.2 and 0.5 g/L for 5, 10, 15, 20 and 30 min and cultured on MS medium supplemented with 1.0 mg/L 2,4-D, 0.5 mg/L NAA and 0.2 mg/L Kin for somatic embryogenesis. Globular embryos induced from the leaf explants were treated with colchicine by immersed method at different concentrations of 0, 0.1, 0.2, 0.3, 0.5 and 0.7% for 24, 36, 48 and 72 h. Then, these embryos were cultured on medium supplemented with 1.0 mg/L 2,4-D, 0.5 mg/L NAA and 0.2 mg/L Kin for secondary embryos induction. The ploidy level was identified by counting chromosomes of the root tip of plantlets derived from these secondary embryos. The results showed that the survival rate and somatic embryo induction the highest (65.56% and 29.89%, respectively) at 0.2 g/L AgNPs for 20 min. For colchicine treatment, the increase in the concentration and exposure time of colchicine decreased the survival rate of explants and the number of secondary embryos but increased the abnormal secondary embryo formation. Identifying the ploidy level showed that the treatments with colchicine concentrations from 0.3% to 0.5% for 48 h lead to tetraploid induction rates from 22.22% to 25.92%. The results also revealed that the tetraploids (2n = 4x = 48) had larger stomatal size, lower stomatal density, denser stomatal chloroplast density, and better growth than did the diploids. 303 111254 2023-11-15T08:53:09Z 2023-11-15T08:53:09Z 2022-09 Journal article Bài báo đăng trên tạp chí thuộc ISI, bao gồm book chapter https://scholar.dlu.edu.vn/handle/123456789/3173 10.1016/j.scienta.2022.111254 vi Scientia Horticulturae 1879-1018 Ahmadi, B., Hamed, E., 2020. In vitro androgenesis: spontaneous vs. artificial genome doubling and characterization of regenerants. Plant Cell Rep 39, 299–316. Allum, J.F., Bringloe, D.H., Roberts, A.V., 2007. Chromosome doubling in a Rosa rugosa Thunb. hybrid by exposure of in vitro nodes to oryzalin: the effects of node length, oryzalin concentration and exposure time. Plant Cell Rep. 26, 1977–1984. Aqafarini, A., Mahmoud, L., Maryam, N., Ghasem, K., 2019. Induction of tetraploidy in garden cress: morphological and cytological changes. Plant Cell Tiss. Organ Cult. 137, 627–635. Banyai, W., Ratchada, S., Netiya, K., Phithak, I., Masahiro, M., Kanyaratt, S., 2010. Overproduction of artemisinin in tetraploid Artemisia annua L. Plant Biotechnol 27, 427–433. Bhusare, B.P., John, C.K., Bhatt, V.P., Nikamb, T.D., 2021. Colchicine induces tetraploids in in vitro cultures of Digitalis lanata Ehrh.: enhanced production of biomass and cardiac glycosides. Ind Crops Prod 174, 114–167. Chau, N.H., Bang, L.A., Buu, N.Q., Dung, T.T.N., Ha, H.T., Quang, D.V., 2008. Some results in manufacturing of nanosilver and investigation of its application for disinfection. Adv Nat Sci 9, 241–248. Comai, L., 2005. The advantages and disadvantages of being polyploid. Nat. Rev. Genet. 6, 836–846. Cuong, D.M., Du, P.C., Tung, H.T., Ngan, H.T.M., Luan, V.Q., Phong, T.H., Khai, H.D., Phuong, T.T.B., Nhut, D.T.N., 2021. Silver nanoparticles as an effective stimulant in micropropagation of Panax vietnamensis-a valuable medicinal plant. Plant Cell Tiss. Organ Cult. 146, 577–588. Demtsu, B., Thunya, T., Msermarl, W., Benya, M., 2013. Induced Mutation by Colchicine Treatment of Somatic Embryos in ‘Namwa’Banana (Musa sp. ABB). ITJEMAST 4, 311–320. Dhooghe, E., Laere, K., Eeckhaut, T., Leus, L., Huylenbroeck, J., 2011. Mitotic chromosome doubling of plant tissues in vitro. Plant Cell Tiss. Organ Cult. 104, 359–373. Duncan, D.B., 1955. Multiple range and multiple F tests. Biometrics 11, 1–42. Eeckhaut, T.G.R., Werbrouck, S.P.O., Leus, L.W.H., Van Bockstaele, E.J., Debergh, P.C., 2004. Chemically Induced Polyploidization in Spathiphyllum wallisii Regel through Somatic Embryogenesis. Plant Cell Tiss. Organ Cult. 78, 241–246. Eng, W., Ho, W., 2019. Polyploidization using colchicine in horticultural plants: a review. Sci. Hortic. 246, 604–617. Fetouh, M.I., Deng, Z., Wilson, S.B., Adams, C.R., Knoxd, G.W., 2020. Induction and characterization of tetraploids in Chinese Privet (Ligustrum sinense Lour.). Sci. Hortic. 271, 109482. Fu, L., Yingying, Z., Min, L., Chunxia, W., Hongmei, S., 2019. Autopolyploid induction via somatic embryogenesis in Lilium distichum Nakai and Lilium cernuum Komar. Plant Cell Tiss. Organ Cult. 139, 237–248. Iannicelli, J., Guariniello, J., Tossi, V.E., Regalado, J.J., Ciaccio, L.D., van Baren, C.M., Alvarez, S.I.P., Escandon, A.S., 2020. The “polyploid effect” in the breeding of aromatic and medicinal species. Sci. Hortic. 260, 108854. Khalili, S., Mohsen, N., Mustafa, A., Maryam, N., 2020. In vitro chromosome doubling of African daisy. Gerbera jamesonii Bolus cv. Mini Red. The Nucleus 63, 59–65. Kim, J.S., Kuk, E., Yu, K.N., Kim, J., Park, S.J., Lee, H.J., Kim, S.H., Park, Y.K., Park, Y.H., Hwany, C.Y., Kim, Y.K., Lee, S.Y., Jeong, D.H., Cho, M.H., 2007. Antimicrobial effects of silver nanoparticles. Nanomedicine 3, 95–101. Kim, K., Nguyen, V.B., Dong, J., Wang, Y., Park, J.Y., Lee, S.C., Yang, T.J., 2017. Evolution of the Araliaceae family inferred from complete chloroplast genomes and 45S nrDNAs of 10 Panax-related species. Sci Rep 7, 4917. Kim, Y.S., Hahn, E.J., Murthy, H.N., Paek, K.Y., 2004. Effect of polyploidy induction on biomass and ginsenoside accumulations in adventitious roots of ginseng. J. Plant Biol. 47, 356–360. Kurtz, L.E., Brand, M.H., Lubell-Brand, J.D., 2020. Production of tetraploid and triploid Hemp. HortScience 55, 1703–1707. Le, K.C., Ho, T.T., Lee, J.D., Paek, K.Y., Park, S.Y., 2020. Colchicine mutagenesis from long-term cultured adventitious roots increases biomass and ginsenoside production in wild ginseng (Panax ginseng Mayer). Agronomy 10, 785. Levin, D.A., Soltis, D.E., 2018. Factors promoting polyploid persistence and diversification and limiting diploid speciation during the K-Pg interlude. Curr. Opin. Plant Biol. 42, 1–7. Li, X., Zhang, Z., Ren, Y., Feng, Y., Guo, Q., Dong, L., Sun, Y., Li, Y., 2021. Induction and early identification of tetraploid black locust by hypocotyl in vitro. In Vitro Cell Dev Biol Plant 57, 372–379. Liao, T., Wang, Y., Xu, C.P., Li, Y., Kang, X.Y., 2018. Adaptive photosynthetic and physiological responses to drought and rewatering in triploid Populus populations. Photosynthetica 56, 578–590. Mondin, M., de Mello el Silva, P.A.K.X., Latado, R.R., Filho, F.A.A.M., 2018. In vitro induction and regeneration of tetraploids and mixoploids of two cassava cultivars. Crop Breed. Appl. Biot. 18, 176–183. Murashige, T., Skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plantarum 15, 473–497. Nakano, A., Mii, M., HoMSino, Y., 2021. Simultaneous production of triploid and hexaploid plants by endosperm culture with colchicine treatment in diploid Haemanthus albiflos. Plant Cell Tiss. Organ Cult. 144, 661–669. Ngan, H.T.M., Cuong, D.M., Tung, H.T., Nghiep, N.D., Le, B.V., Nhut, D.T., 2020. The effect of cobalt and silver nanoparticles on overcoming leaf abscission and enhanced growth of rose (Rosa hybrida L. ‘Baby Love’) plantlets cultured in vitro. Plant Cell Tiss. Organ Cult. 141, 393–405. Nhut, D.T., Huy, N.P., Chien, H.X., 2012a. In vitro culture of petiole longitudinal thin cell layer explants of Vietnamese ginseng (Panax vietnamensis Ha et Grushv.) and preliminary analysis of saponin content. Int. J. Appl. Biol. Pharm. 3, 178. Nhut, D.T., Huy, N.P., Tai, N.T., Nam, N.B., Luan, V.Q., Hien, V.T., Tung, H.T., Vinh, B.V. T., Luan, T.C., 2015. Light-emitting diodes and their potential in callus growth, plantlet development and saponin accumulation during somatic embryogenesis of Panax vietnamensis Ha et Grushv. Biotechnol. Biotechnol. Equip. 29, 299–308. Nhut, D.T., Vinh, B.V.T., Hien, T.T., Huy, N.P., Nam, N.B., Chien, H.X., 2012b. Effects of spermidine, proline and carbohydrate sources on somatic embryogenesis from main root transverse thin cell layers of Vietnamese ginseng (Panax vietnamensis Ha. Grushv.) Afr. J. Biotech. 11, 1084–1091. Niazian, M., Nalousi, A.M., 2020. Artificial polyploidy induction for improvement of ornamental and medicinal plants. Plant Cell Tiss. Organ Cult. 142, 447–469. Niazian, M., Noori, S.A.S., Tohidfar, M., Mortazavian, S.M.M., 2017. Essential oil yield and agro-morphological traits in some Iranian ecotypes of ajowan (Carum copticum L.). J. Essent. Oil-Bear. Plants 20, 1151–1156. Elsevier |