Positive effect of silver nanoparticles in micropropagation of Limonium sinuatum (L.) Mill. ‘White’
The use of silver nanoparticles (AgNPs) is a new direction that promises high economic efficiency in micropropagation of Limonium sinuatum (L.) Mill. ‘White’. In this study, the effect of AgNPs on the explant surface disinfection; in vitro growth, development and acclimatization of L. sinuatum wer...
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| Główni autorzy: | , , , , , , , , , |
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| Format: | Journal article |
| Język: | English |
| Wydane: |
Springer Netherlands
2023
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| Dostęp online: | https://scholar.dlu.edu.vn/handle/123456789/3174 |
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| Thư viện lưu trữ: | Thư viện Trường Đại học Đà Lạt |
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| Streszczenie: | The use of silver nanoparticles (AgNPs) is a new direction that promises high economic efficiency in micropropagation of
Limonium sinuatum (L.) Mill. ‘White’. In this study, the effect of AgNPs on the explant surface disinfection; in vitro growth,
development and acclimatization of L. sinuatum were investigated. Especially, endogenous hormone alterations in shoot
multiplication and rooting stages of plantlets derived from with or without AgNPs (control) treatments were recorded. The
results showed that explants treated with 200 mg/L AgNPs for 20 min not only had a higher disinfection effect but also had
better effects on shoot induction than the explants treated with 1000 mg/L HgCl2
for 5 min. The explant cultured on medium
supplemented with 1.0 mg/L AgNPs showed higher shoot number (5.66 shoots/explant) than the control (2.66 shoots/explant)
via reducing ethylene content (0.690 μg/g) and increasing zeatin content (0.947 μg/g). The rooting time of plantlets was
shortened by 3 days in the medium supplemented with 0.4 mg/L AgNPs as compared to the control, which corresponds to
low ethylene content and high content of IAA, GA3,
and ABA. The results indicated that AgNPs stimulated shoot multiplication
and rooting of L. sinuatum through altering endogenous hormones. Furthermore, 0.4 mg/L AgNPs-derived plantlets
showed well acclimatization as compared to those of the control in greenhouse conditions. |
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