Unveiling Fusaria mycoflora associated with natural occurrence of lisianthus wilt and stem rot in Central-highland Vietnam

Wilt and stem rot (WSR) is an emerging syndrome threatening cut lisianthus (Eustoma russellianum) production in Lam Dong province, Vietnam. The disease was observed in all 13 inspected commercial lisianthus greenhouses across major lisianthus cultivation areas in Lam Dong, including Da Lat, Lac Duon...

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Tác giả chính:	Lê, Dũng
Định dạng:	Journal article
Ngôn ngữ:	English
Được phát hành:	Springer Nature 2024
Những chủ đề:	Fusarium wilt Stem rot F. vanleeuwenii F. annulatum Neocosmospora solani
Truy cập trực tuyến:	https://scholar.dlu.edu.vn/handle/123456789/3590
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id	oai:scholar.dlu.edu.vn:123456789-3590
record_format	dspace
institution	Thư viện Trường Đại học Đà Lạt
collection	Thư viện số
language	English
topic	Fusarium wilt Stem rot F. vanleeuwenii F. annulatum Neocosmospora solani
spellingShingle	Fusarium wilt Stem rot F. vanleeuwenii F. annulatum Neocosmospora solani Lê, Dũng Unveiling Fusaria mycoflora associated with natural occurrence of lisianthus wilt and stem rot in Central-highland Vietnam
description	Wilt and stem rot (WSR) is an emerging syndrome threatening cut lisianthus (Eustoma russellianum) production in Lam Dong province, Vietnam. The disease was observed in all 13 inspected commercial lisianthus greenhouses across major lisianthus cultivation areas in Lam Dong, including Da Lat, Lac Duong, Don Duong, and Duc Trong, with incidence increasing with plant age, ranging from 7.5 to 32.4%. Infected plants displayed stunting, wilting, stem rot and blight, and dieback, with predominance of wilt and stem rot. The disease showed polycyclic behavior, with symptoms shifting from random or scattered in young plants to clustered patterns after the initial flower cutting. Forty-one Fusaria-like fungal isolates recovered from diseased lisianthus plants were identified as Fusarium vanleeuwenii (28 isolates), Neocosmospora solani (11 isolates), and F. annulatum (2 isolates) based on morphological observations and phylogenetic analysis of the internal transcribed spacer (ITS) region and translation elongation factor 1-alpha (TEF-1α) genes. The composition of Fusaria species varied across sites, with F. vanleeuwenii being consistently present. Pathogenicity tests confirmed that isolates of F. vanleeuwenii Li-Fo9511, N. solani Li-Fs4311, and F. annulatum Li-Fp3051 caused typical stem rot in in-vitro assays. In-planta assays showed wilting in seedlings starting two weeks post-infection, with a remarkable increase in disease incidence and severity between five and six weeks, particularly for F. vanleeuwenii Li-Fo9511. The pathogens were re-isolated and morphologically confirmed, fulfilling Koch’s postulates. This is the first report of F. vanleeuwenii, N. solani, and F. annulatum as pathogens of lisianthus WSR in Vietnam, highlighting the need for effective control strategies.
format	Journal article
author	Lê, Dũng
author_facet	Lê, Dũng
author_sort	Lê, Dũng
title	Unveiling Fusaria mycoflora associated with natural occurrence of lisianthus wilt and stem rot in Central-highland Vietnam
title_short	Unveiling Fusaria mycoflora associated with natural occurrence of lisianthus wilt and stem rot in Central-highland Vietnam
title_full	Unveiling Fusaria mycoflora associated with natural occurrence of lisianthus wilt and stem rot in Central-highland Vietnam
title_fullStr	Unveiling Fusaria mycoflora associated with natural occurrence of lisianthus wilt and stem rot in Central-highland Vietnam
title_full_unstemmed	Unveiling Fusaria mycoflora associated with natural occurrence of lisianthus wilt and stem rot in Central-highland Vietnam
title_sort	unveiling fusaria mycoflora associated with natural occurrence of lisianthus wilt and stem rot in central-highland vietnam
publisher	Springer Nature
publishDate	2024
url	https://scholar.dlu.edu.vn/handle/123456789/3590
_version_	1813142643185549312
spelling	oai:scholar.dlu.edu.vn:123456789-35902024-09-29T00:39:51Z Unveiling Fusaria mycoflora associated with natural occurrence of lisianthus wilt and stem rot in Central-highland Vietnam Lê, Dũng Fusarium wilt Stem rot F. vanleeuwenii F. annulatum Neocosmospora solani Wilt and stem rot (WSR) is an emerging syndrome threatening cut lisianthus (Eustoma russellianum) production in Lam Dong province, Vietnam. The disease was observed in all 13 inspected commercial lisianthus greenhouses across major lisianthus cultivation areas in Lam Dong, including Da Lat, Lac Duong, Don Duong, and Duc Trong, with incidence increasing with plant age, ranging from 7.5 to 32.4%. Infected plants displayed stunting, wilting, stem rot and blight, and dieback, with predominance of wilt and stem rot. The disease showed polycyclic behavior, with symptoms shifting from random or scattered in young plants to clustered patterns after the initial flower cutting. Forty-one Fusaria-like fungal isolates recovered from diseased lisianthus plants were identified as Fusarium vanleeuwenii (28 isolates), Neocosmospora solani (11 isolates), and F. annulatum (2 isolates) based on morphological observations and phylogenetic analysis of the internal transcribed spacer (ITS) region and translation elongation factor 1-alpha (TEF-1α) genes. The composition of Fusaria species varied across sites, with F. vanleeuwenii being consistently present. Pathogenicity tests confirmed that isolates of F. vanleeuwenii Li-Fo9511, N. solani Li-Fs4311, and F. annulatum Li-Fp3051 caused typical stem rot in in-vitro assays. In-planta assays showed wilting in seedlings starting two weeks post-infection, with a remarkable increase in disease incidence and severity between five and six weeks, particularly for F. vanleeuwenii Li-Fo9511. The pathogens were re-isolated and morphologically confirmed, fulfilling Koch’s postulates. This is the first report of F. vanleeuwenii, N. solani, and F. annulatum as pathogens of lisianthus WSR in Vietnam, highlighting the need for effective control strategies. 2024-09-26T13:21:10Z 2024-09-26T13:21:10Z 2024-09 Journal article Bài báo đăng trên tạp chí thuộc ISI, bao gồm book chapter https://scholar.dlu.edu.vn/handle/123456789/3590 10.1007/s00203-024-04145-7 en Đề tài cấp Bộ 2023 (Bộ Giáo dục & Đào tạo) Archives of Microbiology 03028933, 1432072X B2023-DLA-04 Bertoldo C, Gilardi G, Spadaro D, Gullino ML, Garibaldi A (2015) Genetic diversity and virulence of Italian strains of Fusarium oxysporum isolated from Eustoma grandiflorum. Eur J Plant Pathol 141:83–97 Bustamante MI, Elfar K, Smith RJ, Bettiga LJ, Tian T, Torres GA et al (2022) First report of Fusarium annulatum associated with young vine decline in California. 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Unveiling Fusaria mycoflora associated with natural occurrence of lisianthus wilt and stem rot in Central-highland Vietnam

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