Micropropagation of an endangered orchid Anoectochilus formosanus
A rapid and efficient procedure is outlined for in vitro clonal propagation of an elite cultivar of jewel orchid (Anoectochilus formosanus). Multiple shoot proliferation was induced in shoot tip explants on Hyponex (H3) media supplemented with 1 mg dm-3 benzyladenine or 1 - 2 mg dm-3 thidiazuron (T...
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| Định dạng: | Journal article |
| Ngôn ngữ: | English |
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Institute of Experimental Botany, Czech Academy of Sciences
2022
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| Truy cập trực tuyến: | http://scholar.dlu.edu.vn/handle/123456789/885 |
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oai:scholar.dlu.edu.vn:123456789-8852022-09-09T08:32:04Z Micropropagation of an endangered orchid Anoectochilus formosanus Nguyễn, Văn Kết EJ, Hahn SY, Park D, Chakrabarty KY, Paek activated charcoal benzyladenine sugar thidiazuron A rapid and efficient procedure is outlined for in vitro clonal propagation of an elite cultivar of jewel orchid (Anoectochilus formosanus). Multiple shoot proliferation was induced in shoot tip explants on Hyponex (H3) media supplemented with 1 mg dm-3 benzyladenine or 1 - 2 mg dm-3 thidiazuron (TDZ). Addition of activated charcoal (1 g dm-3) to the TDZ containing medium promoted multiple shoot formation (11.1 shoots per explant). However, the regenerated shoots had slow growth rate and failed to elongate. This problem was overcome by transferring the shoot clumps to a hormone free H3 medium supplemented with 2 % sucrose and 0.5 g dm-3 activated charcoal. Rooting was induced in 100 % of the regenerated shoots in the same media. The plantlets were acclimatized and established in greenhouse. 48 3 339-344 2022-09-09T08:32:01Z 2022-09-09T08:32:01Z 2004 Journal article Bài báo đăng trên tạp chí thuộc ISI, bao gồm book chapter 0006-3134 http://scholar.dlu.edu.vn/handle/123456789/885 en Biologia plantarum Institute of Experimental Botany, Czech Academy of Sciences Czech Repulic |
| institution |
Thư viện Trường Đại học Đà Lạt |
| collection |
Thư viện số |
| language |
English |
| topic |
activated charcoal benzyladenine sugar thidiazuron |
| spellingShingle |
activated charcoal benzyladenine sugar thidiazuron Nguyễn, Văn Kết EJ, Hahn SY, Park D, Chakrabarty KY, Paek Micropropagation of an endangered orchid Anoectochilus formosanus |
| description |
A rapid and efficient procedure is outlined for in vitro clonal propagation of an elite cultivar of jewel orchid
(Anoectochilus formosanus). Multiple shoot proliferation was induced in shoot tip explants on Hyponex (H3) media supplemented with 1 mg dm-3 benzyladenine or 1 - 2 mg dm-3 thidiazuron (TDZ). Addition of activated charcoal (1 g dm-3) to the TDZ containing medium promoted multiple shoot formation (11.1 shoots per explant). However, the regenerated shoots had slow growth rate and failed to elongate. This problem was overcome by transferring the shoot clumps to a hormone free H3 medium supplemented with 2 % sucrose and 0.5 g dm-3 activated charcoal. Rooting was induced in 100 % of the regenerated shoots in the same media. The plantlets were acclimatized and established in greenhouse. |
| format |
Journal article |
| author |
Nguyễn, Văn Kết EJ, Hahn SY, Park D, Chakrabarty KY, Paek |
| author_facet |
Nguyễn, Văn Kết EJ, Hahn SY, Park D, Chakrabarty KY, Paek |
| author_sort |
Nguyễn, Văn Kết |
| title |
Micropropagation of an endangered orchid Anoectochilus formosanus |
| title_short |
Micropropagation of an endangered orchid Anoectochilus formosanus |
| title_full |
Micropropagation of an endangered orchid Anoectochilus formosanus |
| title_fullStr |
Micropropagation of an endangered orchid Anoectochilus formosanus |
| title_full_unstemmed |
Micropropagation of an endangered orchid Anoectochilus formosanus |
| title_sort |
micropropagation of an endangered orchid anoectochilus formosanus |
| publisher |
Institute of Experimental Botany, Czech Academy of Sciences |
| publishDate |
2022 |
| url |
http://scholar.dlu.edu.vn/handle/123456789/885 |
| _version_ |
1768305882514325504 |