Silver nanoparticles as the sterilant in large-scale micropropagation of chrysanthemum
Micropropagation has proven to be an effective method for large-scale plant production in a short time and a useful tool for plant breeding. Microbial contamination is one of the most difficult micropropagation challenges, resulting in reduced plant quality and loss of valuable stocks. Therefore, st...
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2022
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oai:scholar.dlu.edu.vn:123456789-9402022-10-12T01:45:01Z Silver nanoparticles as the sterilant in large-scale micropropagation of chrysanthemum Hoang Thanh Tung Huynh Gia Bao Do Manh Cuong Ha Thi My Ngan Vu Thi Hien Vu Quoc Luan Bui Van The Vinh Hoàng, Thị Như Phương Nguyễn, Bá Nam Lê, Ngọc Triệu Nguyễn, Khoa Trưởng Pham Nguyen Duc Hoang Duong Tan Nhut Bacteria Explant surface disinfection Fungi Large-scale micropropagation systems Silver nanoparticles Non-sterilized medium Micropropagation has proven to be an effective method for large-scale plant production in a short time and a useful tool for plant breeding. Microbial contamination is one of the most difficult micropropagation challenges, resulting in reduced plant quality and loss of valuable stocks. Therefore, sterilization of culture media is a critical step in plant micropropagation. However, sterilized media might reduce the activity of plant growth regulators and nutritional components of culture media. The sterilization effects of silver nanoparticles (AgNP) on the growth of explants and culture media were examined. The treatment with 250 ppm AgNP for 15 to 20 min of 4-wk-old ex vitro leaves proved optimal for controlling the contamination. Furthermore, the Murashige and Skoog medium containing 4 ppm AgNP resulted in 100% medium disinfection (no contamination) after 4 wk of culture. The plantlets obtained from non-sterilized MS medium (NoM) containing 4 ppm AgNP and 4 g L−1 agar gave similar results as the control medium with 8 g L−1 agar and the absence of AgNP. Large scale culture systems using NoM in large plastic containers of two different sizes (NoM1 and NoM2) could produce quality plantlets. Chrysanthemum plantlets in the NoM1 system showed higher antioxidant enzyme activities of ascorbate peroxidase and superoxide dismutase than plantlets in the autoclaved medium. Furthermore, the plantlets from NoM were better acclimatized under greenhouse conditions than those from the autoclaved medium (AuM) system. The developmental stages (flower buds and blooming time) of NoM1 and NoM2 plantlets, were 1 wk earlier than those from the AuM system. The successful use of AgNP as a sterilizer and as a component of culture media would reduce the cost of micropropagation and improve plants' quality. Published online 15 March 2021 2022-09-13T09:36:26Z 2022-09-13T09:36:26Z 2021 Journal article Bài báo đăng trên tạp chí thuộc ISI, bao gồm book chapter http://scholar.dlu.edu.vn/handle/123456789/940 https://doi.org/10.1007/s11627-021-10163-7 en Plant cell tissue culture Springer 2021 |
| institution |
Thư viện Trường Đại học Đà Lạt |
| collection |
Thư viện số |
| language |
English |
| topic |
Bacteria Explant surface disinfection Fungi Large-scale micropropagation systems Silver nanoparticles Non-sterilized medium |
| spellingShingle |
Bacteria Explant surface disinfection Fungi Large-scale micropropagation systems Silver nanoparticles Non-sterilized medium Hoang Thanh Tung Huynh Gia Bao Do Manh Cuong Ha Thi My Ngan Vu Thi Hien Vu Quoc Luan Bui Van The Vinh Hoàng, Thị Như Phương Nguyễn, Bá Nam Lê, Ngọc Triệu Nguyễn, Khoa Trưởng Pham Nguyen Duc Hoang Duong Tan Nhut Silver nanoparticles as the sterilant in large-scale micropropagation of chrysanthemum |
| description |
Micropropagation has proven to be an effective method for large-scale plant production in a short time and a useful tool for plant breeding. Microbial contamination is one of the most difficult micropropagation challenges, resulting in reduced plant quality and loss of valuable stocks. Therefore, sterilization of culture media is a critical step in plant micropropagation. However, sterilized media might reduce the activity of plant growth regulators and nutritional components of culture media. The sterilization effects of silver nanoparticles (AgNP) on the growth of explants and culture media were examined. The treatment with 250 ppm AgNP for 15 to 20 min of 4-wk-old ex vitro leaves proved optimal for controlling the contamination. Furthermore, the Murashige and Skoog medium containing 4 ppm AgNP resulted in 100% medium disinfection (no contamination) after 4 wk of culture. The plantlets obtained from non-sterilized MS medium (NoM) containing 4 ppm AgNP and 4 g L−1 agar gave similar results as the control medium with 8 g L−1 agar and the absence of AgNP. Large scale culture systems using NoM in large plastic containers of two different sizes (NoM1 and NoM2) could produce quality plantlets. Chrysanthemum plantlets in the NoM1 system showed higher antioxidant enzyme activities of ascorbate peroxidase and superoxide dismutase than plantlets in the autoclaved medium.
Furthermore, the plantlets from NoM were better acclimatized under greenhouse conditions than those from the autoclaved medium (AuM) system. The developmental stages (flower buds and blooming time) of NoM1 and NoM2 plantlets, were 1 wk earlier than those from the AuM system. The successful use of AgNP as a sterilizer and as a component of culture media would reduce the cost of micropropagation and improve plants' quality. |
| format |
Journal article |
| author |
Hoang Thanh Tung Huynh Gia Bao Do Manh Cuong Ha Thi My Ngan Vu Thi Hien Vu Quoc Luan Bui Van The Vinh Hoàng, Thị Như Phương Nguyễn, Bá Nam Lê, Ngọc Triệu Nguyễn, Khoa Trưởng Pham Nguyen Duc Hoang Duong Tan Nhut |
| author_facet |
Hoang Thanh Tung Huynh Gia Bao Do Manh Cuong Ha Thi My Ngan Vu Thi Hien Vu Quoc Luan Bui Van The Vinh Hoàng, Thị Như Phương Nguyễn, Bá Nam Lê, Ngọc Triệu Nguyễn, Khoa Trưởng Pham Nguyen Duc Hoang Duong Tan Nhut |
| author_sort |
Hoang Thanh Tung |
| title |
Silver nanoparticles as the sterilant in large-scale micropropagation of chrysanthemum |
| title_short |
Silver nanoparticles as the sterilant in large-scale micropropagation of chrysanthemum |
| title_full |
Silver nanoparticles as the sterilant in large-scale micropropagation of chrysanthemum |
| title_fullStr |
Silver nanoparticles as the sterilant in large-scale micropropagation of chrysanthemum |
| title_full_unstemmed |
Silver nanoparticles as the sterilant in large-scale micropropagation of chrysanthemum |
| title_sort |
silver nanoparticles as the sterilant in large-scale micropropagation of chrysanthemum |
| publisher |
Springer |
| publishDate |
2022 |
| url |
http://scholar.dlu.edu.vn/handle/123456789/940 https://doi.org/10.1007/s11627-021-10163-7 |
| _version_ |
1768305900680904704 |