Micropropagation of Jasminanthes tuyetanhiae: an endemic and valuable herb in Vietnam
Jasminanthes tuyetanhiae is one of the precious herbs and good for human health, discovered in the Da-Bia Mountain (Phu Yen province, Vietnam). The identification of the CTW species (species believed to be J. tuyetanhiae collected in 2019) by molecular indicators showed that this species belongs to...
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2022
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| Truy cập trực tuyến: | http://scholar.dlu.edu.vn/handle/123456789/956 https://doi.org/10.1007/s11240-021-02158-4 |
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oai:scholar.dlu.edu.vn:123456789-9562022-10-15T01:44:44Z Micropropagation of Jasminanthes tuyetanhiae: an endemic and valuable herb in Vietnam Nguyễn, Bá Nam Lê, Ngọc Triệu Vu, Nguyen Tran Trung, Le Huy Tra, Trinh Thi Huy Tram, Le Trinh Ngoc Phan, Hoàng Đại Tung, Hoang Thanh Nhut, Duong Tan Jasminanthes tuyetanhiae ITS1-5.8SrRNA-ITS2 matK Micro-propagation Ventilation Jasminanthes tuyetanhiae is one of the precious herbs and good for human health, discovered in the Da-Bia Mountain (Phu Yen province, Vietnam). The identification of the CTW species (species believed to be J. tuyetanhiae collected in 2019) by molecular indicators showed that this species belongs to the Apocynaceae family. High similarity (98.2%) in the ITS1-5.8SrRNA-ITS2 gene sequence of CTW when compared on Genbank with J. tuyetanhiae and genetic distance difference (1.3%) - in the limit of difference of the same taxon considered on matK sequence shows that CTW is likely to belong to this species. Surface sterilization of ex vitro explants with HgCl2 (0.1%) for 3 min gave the highest sterilization efficiency compared to the remaining treatments with the induction explant rate of 93.33%. BA (0.5 mg l−1) is the most optimal for effective in vitro shoot multiplication in this species. Adding IBA to culture media at the appropriate concentration (0.5 mg l−1) increased rooting ability, shortened rooting time (13 days) and increased root length (6.53 mm). The ventilation culture system improves rooting efficiency, stimulates growth and induces secondary root formation—which is a suitable system for in vitro propagation of the Jasminanthes tuyetanhiae in this study. Published online 30 August 2021 2022-09-14T02:22:55Z 2022-09-14T02:22:55Z 2021 Journal article Bài báo đăng trên tạp chí thuộc ISI, bao gồm book chapter 1573-5044 http://scholar.dlu.edu.vn/handle/123456789/956 10.1007/s11240-021-02158-4 https://doi.org/10.1007/s11240-021-02158-4 en Plant Cell, Tissue and Organ Culture 15735044, 01676857 Springer |
| institution |
Thư viện Trường Đại học Đà Lạt |
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| language |
English |
| topic |
Jasminanthes tuyetanhiae ITS1-5.8SrRNA-ITS2 matK Micro-propagation Ventilation |
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Jasminanthes tuyetanhiae ITS1-5.8SrRNA-ITS2 matK Micro-propagation Ventilation Nguyễn, Bá Nam Lê, Ngọc Triệu Vu, Nguyen Tran Trung, Le Huy Tra, Trinh Thi Huy Tram, Le Trinh Ngoc Phan, Hoàng Đại Tung, Hoang Thanh Nhut, Duong Tan Micropropagation of Jasminanthes tuyetanhiae: an endemic and valuable herb in Vietnam |
| description |
Jasminanthes tuyetanhiae is one of the precious herbs and good for human health, discovered in the Da-Bia Mountain (Phu Yen province, Vietnam). The identification of the CTW species (species believed to be J. tuyetanhiae collected in 2019) by molecular indicators showed that this species belongs to the Apocynaceae family. High similarity (98.2%) in the ITS1-5.8SrRNA-ITS2 gene sequence of CTW when compared on Genbank with J. tuyetanhiae and genetic distance difference (1.3%) - in the limit of difference of the same taxon considered on matK sequence shows that CTW is likely to belong to this species. Surface sterilization of ex vitro explants with HgCl2 (0.1%) for 3 min gave the highest sterilization efficiency compared to the remaining treatments with the induction explant rate of 93.33%. BA (0.5 mg l−1) is the most optimal for effective in vitro shoot multiplication in this species. Adding IBA to culture media at the appropriate concentration (0.5 mg l−1) increased rooting ability, shortened rooting time (13 days) and increased root length (6.53 mm). The ventilation culture system improves rooting efficiency, stimulates growth and induces secondary root formation—which is a suitable system for in vitro propagation of the Jasminanthes tuyetanhiae in this study. |
| format |
Journal article |
| author |
Nguyễn, Bá Nam Lê, Ngọc Triệu Vu, Nguyen Tran Trung, Le Huy Tra, Trinh Thi Huy Tram, Le Trinh Ngoc Phan, Hoàng Đại Tung, Hoang Thanh Nhut, Duong Tan |
| author_facet |
Nguyễn, Bá Nam Lê, Ngọc Triệu Vu, Nguyen Tran Trung, Le Huy Tra, Trinh Thi Huy Tram, Le Trinh Ngoc Phan, Hoàng Đại Tung, Hoang Thanh Nhut, Duong Tan |
| author_sort |
Nguyễn, Bá Nam |
| title |
Micropropagation of Jasminanthes tuyetanhiae: an endemic and valuable herb in Vietnam |
| title_short |
Micropropagation of Jasminanthes tuyetanhiae: an endemic and valuable herb in Vietnam |
| title_full |
Micropropagation of Jasminanthes tuyetanhiae: an endemic and valuable herb in Vietnam |
| title_fullStr |
Micropropagation of Jasminanthes tuyetanhiae: an endemic and valuable herb in Vietnam |
| title_full_unstemmed |
Micropropagation of Jasminanthes tuyetanhiae: an endemic and valuable herb in Vietnam |
| title_sort |
micropropagation of jasminanthes tuyetanhiae: an endemic and valuable herb in vietnam |
| publisher |
Springer |
| publishDate |
2022 |
| url |
http://scholar.dlu.edu.vn/handle/123456789/956 https://doi.org/10.1007/s11240-021-02158-4 |
| _version_ |
1768305906164957184 |