Extracellular matrix protocols

The study of the extracellular matrix has significantly expanded over the past decade. Although thoroughly researched, the complexity and the dynamic nature of the matrix are still under extensive investigation, yielding a growing number of methods of analysis. In Extracellular Matrix Prools: Second...

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Những tác giả chính:	Ram, Sharona Even, Artym, Vira V
Định dạng:	Sách
Ngôn ngữ:	English
Được phát hành:	Humana 2012
Những chủ đề:	Extracellular matrix Recombinant retrovirus ,
Truy cập trực tuyến:	http://scholar.dlu.edu.vn/thuvienso/handle/DLU123456789/31954
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id	oai:scholar.dlu.edu.vn:DLU123456789-31954
record_format	dspace
institution	Thư viện Trường Đại học Đà Lạt
collection	Thư viện số
language	English
topic	Extracellular matrix Recombinant retrovirus ,
spellingShingle	Extracellular matrix Recombinant retrovirus , Ram, Sharona Even Artym, Vira V Extracellular matrix protocols
description	The study of the extracellular matrix has significantly expanded over the past decade. Although thoroughly researched, the complexity and the dynamic nature of the matrix are still under extensive investigation, yielding a growing number of methods of analysis. In Extracellular Matrix Prools: Second Edition, expert researchers explore this developing field and provide a number of techniques which can be reproduced in most cell biology labs. This edition contains methods that represent cutting edge unique technologies which are well worth exposure to the broad field and may encourage collaboration with expert labs. Chapters include information on molecular biology techniques to study gene expression, biophysical and biochemical methods for the analysis of structure and composition, and cell biology methods for assessment of cell behavior, matrix assembly and tissue engineering applications. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Extracellular Matrix Prools: Second Edition investigates the diverse roles of the extracellular matrix, providing important tools for all researchers in the ECM community.
format	Book
author	Ram, Sharona Even Artym, Vira V
author_facet	Ram, Sharona Even Artym, Vira V
author_sort	Ram, Sharona Even
title	Extracellular matrix protocols
title_short	Extracellular matrix protocols
title_full	Extracellular matrix protocols
title_fullStr	Extracellular matrix protocols
title_full_unstemmed	Extracellular matrix protocols
title_sort	extracellular matrix protocols
publisher	Humana
publishDate	2012
url	http://scholar.dlu.edu.vn/thuvienso/handle/DLU123456789/31954
_version_	1757676031096913920
spelling	oai:scholar.dlu.edu.vn:DLU123456789-319542014-01-20T04:19:52Z Extracellular matrix protocols Ram, Sharona Even Artym, Vira V Extracellular matrix Recombinant retrovirus , The study of the extracellular matrix has significantly expanded over the past decade. Although thoroughly researched, the complexity and the dynamic nature of the matrix are still under extensive investigation, yielding a growing number of methods of analysis. In Extracellular Matrix Prools: Second Edition, expert researchers explore this developing field and provide a number of techniques which can be reproduced in most cell biology labs. This edition contains methods that represent cutting edge unique technologies which are well worth exposure to the broad field and may encourage collaboration with expert labs. Chapters include information on molecular biology techniques to study gene expression, biophysical and biochemical methods for the analysis of structure and composition, and cell biology methods for assessment of cell behavior, matrix assembly and tissue engineering applications. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Extracellular Matrix Prools: Second Edition investigates the diverse roles of the extracellular matrix, providing important tools for all researchers in the ECM community. Preface v Contributors ix Part I Molecular Biology 1 Retroviral Delivery of ECM Genes Vitali Alexeev Olga Igoucheva 3 2 Tissue-Specific KO of ECM Proteins Mara Brancaccio Emila Turco Emilio Hirsch 15 3 Recombinant Collagen Trimers from Insect Cells and Yeast Johanna Myllyharju 51 4 Eukaryotic Expression and Purification of Recombinant Extracellular Matrix Proteins Carrying the Strep II Tag Neil Smyth Uwe Odenthal Barbara Merkl Mats Paulsson 63 5 Preparation of Recombinant Fibronectin Fragments for Functional and Structural Studies David Staunton Christopher J. Millard A. Radu Aricescu Iain D. Campbell 73 Part II Biochemical and Biophysical Analysis 6 Quantitative Determination of Collagen Cross-links Nicholas C. Avery Trevor J. Sims Allen J. Bailey 103 7 ECM Macromolecules: Height-Mapping and Nano-Mechanics Using Atomic Force Microscopy Nigel W. Hodson Cay M. Kielty Michael J. Sherratt 123 8 Atomic Force Microscopy Measurements of Intermolecular Binding Forces Gradimir N. Misevic Yannis Karamanos Nikola J. Misevic 143 9 Mass-Mapping of ECM Macromolecules by Scanning Transmission Electron Microscopy Michael J. Sherratt Helen K. Graham Cay M. Kielty David F. Holmes 151 10 Chemical Microscopy of Biological Samples by Dynamic Mode Secondary Ion Mass Spectrometry Gradimir N. Misevic Bernard Rasser Vic Norris Cédric Dérue David Gibouin Fabrice Lefebvre Marie-Claire Verdus Anthony Delaune Guillaume Legent Camille Ripoll 163 11 ECM Macromolecules: Rotary Shadowing and Transmission Electron Microscopy Michael J. Sherratt Roger S. Meadow Helen K. Graham Cay M. Kielty David F. Holmes 175 12 Using Self-Assembled Monolayers to Pattern ECM Proteinsand Cells on Substrates Emanuele Ostuni George M. Whitesides Donald E. Ingber Christopher S. Chen 183 13 Solid Phase Assays for Studying ECM Protein-Protein Interactions A Paul Mould 195 Part III Cell Biology Assays 14 Cell Adhesion Assays Martin J. Humphries 203 15 ECM Degradation Assays for Analyzing Local Cell Invasion Vira V. Artym Kenneth M. Yamada Susette C. Mueller 211 16 Fluorescence-Based Assays for In Vitro Analysis of Cell Adhesion and Migration Paola Spessotto Katia Lacrima Pier Andrea Nicolosi Eliana Pivetta Martina Scapolan Roberto Perris 221 17 Fibrin Gel Model for Assessment of Cellular Contractility Sharona Even-Ram 251 18 Fluorescent Labeling Techniques for Investigation of Fibronectin Fibrillogenesis (Labeling Fibronectin Fibrillogenesis) Roumen Pankov Albena Momchilova 261 19 Stromagenesis During Tumorigenesis: Characterization of Tumor-Associated Fibroblasts and Stroma-Derived 3D Matrices Remedios Castelló-Cros Edna Cukierman 275 Part IV Organ Models 20 Tissue Recombinants to Study Extracellular Matrix Targeting to Basement Membranes Patricia Simon-Assmann Anne-Laure Bolcato-Bellemin Annick Klein Michèle Kedinger 309 21 ECM and FGF-Dependent Assay of Embryonic SMG Epithelial Morphogenesis: Investigating Growth Factor/Matrix Regulation of Gene Expression During Submandibular Gland Development Ivan T. Rebustini Matthew P. Hoffman 319 22 Analyzing how Cell Adhesion Controls Mammary Gland Function by Transplantation of Embryonic Mammary Tissue from Knockout Mice Teresa C.M. Klinowska Charles H. Streuli 331 Part V Tissue Engineering 23 Characterizing ECM Production by Cells Encapsulated in Hydrogels Iossif A. Strehin Jennifer H. Elisseeff 349 24 Tissue Engineering and Cell-Populated Collagen Matrices Paul D. Kemp 363 Index 371 2012-09-19T01:37:27Z 2012-09-19T01:37:27Z 2009 Book http://scholar.dlu.edu.vn/thuvienso/handle/DLU123456789/31954 en application/pdf Humana

Extracellular matrix protocols

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