Annual plant reviews, Volume 28: Plant Proteomics
The proteome comprises all protein species resulting from gene expression in a cell, organelle, tissue or organism. By definition, proteomics aims to identify and characterise the expression pattern, cellular location, activity, regulation, post-translational modifications, molecular interactions, t...
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Blackwell
2012
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Thư viện Trường Đại học Đà Lạt |
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English |
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Plant proteins Plant proteomics |
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Plant proteins Plant proteomics Finnie, Christine Annual plant reviews, Volume 28: Plant Proteomics |
| description |
The proteome comprises all protein species resulting from gene expression in a cell, organelle, tissue or organism. By definition, proteomics aims to identify and characterise the expression pattern, cellular location, activity, regulation, post-translational modifications, molecular interactions, three dimensional structures and functions of each protein in a biological system. In plant science, the number of proteome studies is rapidly expanding after the completion of the Arabidopsis thaliana genome sequence, and proteome analyses of other important or emerging model systems and crop plants are in progress or are being initiated. Proteome analysis in plants is subject to the same obstacles and limitations as in other organisms, but the nature of plant tissues, with their rigid cell walls and complex variety of secondary metabolites, means that extra challenges are involved that may not be faced when analysing other organisms. This volume aims to highlight the ways in which proteome analysis has been used to probe the complexities of plant biochemistry and physiology. It is aimed at researchers in plant biochemistry, genomics, transcriptomics and metabolomics who wish to gain an up-to-date insight into plant proteomes, the information plant proteomics can yield and the directions plant proteome research is taking. |
| format |
Book |
| author |
Finnie, Christine |
| author_facet |
Finnie, Christine |
| author_sort |
Finnie, Christine |
| title |
Annual plant reviews, Volume 28: Plant Proteomics |
| title_short |
Annual plant reviews, Volume 28: Plant Proteomics |
| title_full |
Annual plant reviews, Volume 28: Plant Proteomics |
| title_fullStr |
Annual plant reviews, Volume 28: Plant Proteomics |
| title_full_unstemmed |
Annual plant reviews, Volume 28: Plant Proteomics |
| title_sort |
annual plant reviews, volume 28: plant proteomics |
| publisher |
Blackwell |
| publishDate |
2012 |
| url |
http://scholar.dlu.edu.vn/thuvienso/handle/DLU123456789/31984 |
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1757674055305003008 |
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oai:scholar.dlu.edu.vn:DLU123456789-319842014-01-20T04:18:05Z Annual plant reviews, Volume 28: Plant Proteomics Finnie, Christine Plant proteins Plant proteomics The proteome comprises all protein species resulting from gene expression in a cell, organelle, tissue or organism. By definition, proteomics aims to identify and characterise the expression pattern, cellular location, activity, regulation, post-translational modifications, molecular interactions, three dimensional structures and functions of each protein in a biological system. In plant science, the number of proteome studies is rapidly expanding after the completion of the Arabidopsis thaliana genome sequence, and proteome analyses of other important or emerging model systems and crop plants are in progress or are being initiated. Proteome analysis in plants is subject to the same obstacles and limitations as in other organisms, but the nature of plant tissues, with their rigid cell walls and complex variety of secondary metabolites, means that extra challenges are involved that may not be faced when analysing other organisms. This volume aims to highlight the ways in which proteome analysis has been used to probe the complexities of plant biochemistry and physiology. It is aimed at researchers in plant biochemistry, genomics, transcriptomics and metabolomics who wish to gain an up-to-date insight into plant proteomes, the information plant proteomics can yield and the directions plant proteome research is taking. Cover -- Contents -- Preface -- Contributors -- 1 Plant proteomics58; challenges and resources -- 146;1 Introduction -- 146;2 Challenges -- 146;246;1 Sample extraction -- 146;246;2 Sample preparation and arraying -- 146;246;3 Mass spectrometry 40;MALDI and ESI41; -- 146;246;4 Analysis depth -- 146;246;5 Data analysis -- 146;246;6 Quantitation -- 146;246;7 Modifications -- 146;246;8 Data -- 146;3 Resources -- 146;346;1 Proteomic databases -- 146;346;2 Online proteomic tools and resources -- 146;4 Future -- 2 Proteomic analysis of post45;translational modifications by mass spectrometry -- 246;1 Summary -- 246;2 Introduction -- 246;3 Considerations for the experimental design of PTM analysis by proteomics -- 246;4 Analysis of PTMs by proteomic approaches -- 246;446;1 Phosphorylation -- 246;446;2 Protein glycosylation -- 246;446;3 GPI45;AP -- 246;446;4 Farnesylation -- 246;446;5 N45;terminally modified proteins -- 246;5 Conclusions and perspectives -- 3 Strategies for the investigation of protein8211;protein interactions in plants -- 346;1 Summary -- 346;2 Introduction -- 346;3 Biochemical procedures to characterize protein8211;protein interactions -- 346;346;1 Chromatographic purifications -- 346;346;2 Sucrose gradient ultrafiltration -- 346;346;3 Native gel electrophoresis -- 346;346;4 Immunoprecipitations -- 346;4 Genetic procedures to characterize protein8211;protein interactions -- 346;446;1 Yeast two45;hybrid system -- 346;446;2 Yeast three45;hybrid system -- 346;446;3 Yeast one45;hybrid system -- 346;446;4 Limitations of yeast two45;hybrid systems -- 346;446;5 Split45;ubiquitin system -- 346;446;6 Bimolecular fluorescence complementation 40;BiFC41; -- 346;446;7 F246;rster resonance energy transfer 40;FRET41; -- 346;446;8 Tagging technologies for the purification of protein complexes -- 346;5 Cytological procedures to characterize protein8211;protein interactions -- 346;6 Outlook -- 4 Proteomics of disulphide and cysteine oxidoreduction -- 446;1 Introduction -- 446;2 Control of cellular redox status -- 446;246;1 Sequence and structural features of proteins catalysing cysteine redox modifications -- 446;246;2 Catalytic mechanisms of Trxs and Grxs -- 446;3 Proteomics techniques for analysis of cysteine modifications -- 446;346;1 Reagents for cysteine labelling -- 446;346;2 Disulphide mapping -- 446;346;3 S45;glutathionylation -- 446;346;4 Cysteine SOH44; SO[sub40;241;]H and SO[sub40;341;]H -- 446;346;5 Trxs and disulphide reduction -- 446;346;6 S45;nitrosylation -- 446;4 Conclusions and perspectives -- 5 Structural proteomics -- 546;1 Introduction -- 546;2 Project data handling58; Sesame -- 546;3 ORF cloning -- 546;4 E46; coli cell45;based protein production pipeline -- 546;446;1 Large45;scale protein production and labeling -- 546;446;2 Protein purification -- 546;5 Wheat germ cell45;free protein production -- 546;6 Mass spectrometry of purified proteins for quality assurance and analysis -- 546;7 Crystallomics and X45;ray structure determination -- 546;746;1 Initial screening -- 546;746;2 Optimizations and salvage -- 546;746;3 Future directions -- 546;8 NMR screening and structure determination -- 546;846;1 Sample. 2012-09-19T08:51:49Z 2012-09-19T08:51:49Z 2006 Book http://scholar.dlu.edu.vn/thuvienso/handle/DLU123456789/31984 en application/pdf Blackwell |