Seeds: Biology, Development and Ecology
Desiccation tolerance (DT), or the capacity to survive nearly complete drying, is considered to be a multifactorial trait, involving the protection of macromolecules against water removal and regulation of the metabolism to prevent oxidative stress and the production of by-products to toxic level...
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| Những tác giả chính: | , , |
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| 格式: | 图书 |
| 语言: | English |
| 出版: |
CABI
2014
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| 主题: | |
| 在线阅读: | https://scholar.dlu.edu.vn/thuvienso/handle/DLU123456789/36417 |
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| Thư viện lưu trữ: | Thư viện Trường Đại học Đà Lạt |
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| 总结: | Desiccation tolerance (DT), or the capacity to survive nearly complete drying, is considered
to be a multifactorial trait, involving the protection of macromolecules against water
removal and regulation of the metabolism to prevent oxidative stress and the production of
by-products to toxic levels. To obtain a general overview of the processes, molecules and
regulating pathways involved in the acquisition of DT, transcriptome profiling was carried
out on desiccation-tolerant and desiccation-sensitive stages of Medicago truncatula Gaertn.
seeds using 16K microarrays. In order to discriminate DT from other developmental pathways,
differences in gene expression were determined for two different stages: (i) between
desiccation-sensitive embryos at 14 days after pollination (DAP) and those that had acquired
their DT at 20 DAP; and (ii) between desiccation-sensitive radicles of germinated seeds and
radicles of those germinated seeds that had received an osmotic treatment, resulting in the
re-establishment of DT. Functional analysis of genes that were at least twofold upregulated
or downregulated in both desiccation-tolerant stages revealed that the main groups containing
upregulated genes belonged to defence and seed storage reserves. Groups containing
typically downregulated genes were those involved in cell cycle and DNA processing as well
as primary and energy metabolism. The main findings are discussed and compared with
other desiccation-tolerant organisms. |
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