Cloning of genes coding for L-sorbose and L-sorbosone dehydrogenases from Gluconobacter oxydans and microbial production of 2-keto-L- gulonate, a precursor of L-ascorbic acid, in a recombinant G. oxydans strain /

Míreanna Comhchosúla

• Direct fermentation of 2-keto-l-gulonic acid in recombinant Gluconobacter oxydans /
• 5-Keto-D-gluconate production is catalyzed by a quinoprotein glycerol dehydrogenase, major polyol dehydrogenase, in gluconobacter species /
• Chloroperoxidase-catalyzed enantioselective oxidation of methyl phenyl sulfide with dihydroxyfumaric acid/oxygen or ascorbic acid/oxygen as oxidants /
• Can the combination of electrochemical regeneration of NAD+, selectivity of L-alpha-amino-acid dehydrogenase, and reductive amination of alpha-keto-acid be applied to the inversion of configuration of a L-alpha-amino-acid ? /
• Functional replacement of the escherichia coli D-(-)-lactate dehydrogenase gene (ldhA) with the L-(+)-lactate dehydrogenase gene (ldhL) from pediococcus acidilactici /
  le: Zhou, Shengde.