Somatic embryogenesis through pseudo-bulblet transverse thin cell layer of Lilium longiflorum

Somatic embryogenesis was achieved directly from pseudo-bulblettransverse thin cell layers (tTCL) of Lilium longiflorum.Embryo-like structures (globular embryos) were obtained from different sizeexplants of pseudo-bulblet tTCLs after 45 days culture on Murashige and Skoog,1962 (MS) medium containing...

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Những tác giả chính: Duong Tan Nhut, Bui Van Le, Nguyễn, Trí Minh, Jaime Teixeira de Silva, Seiichi Fukai, Michio Tanaka, K. Tran Thanh Van
Định dạng: Journal article
Ngôn ngữ:Vietnamese
Được phát hành: Springer 2023
Những chủ đề:
Truy cập trực tuyến:https://scholar.dlu.edu.vn/handle/123456789/2629
Các nhãn: Thêm thẻ
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Thư viện lưu trữ: Thư viện Trường Đại học Đà Lạt
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Tóm tắt:Somatic embryogenesis was achieved directly from pseudo-bulblettransverse thin cell layers (tTCL) of Lilium longiflorum.Embryo-like structures (globular embryos) were obtained from different sizeexplants of pseudo-bulblet tTCLs after 45 days culture on Murashige and Skoog,1962 (MS) medium containing 5.4 μM naphthalene acetic acid(NAA)and 1.1 μM thidiazuron (TDZ). The embryo-like structures werethen isolated and mass proliferated on MS medium, containing 5.4μM NAA and 0.4 μM TDZ, every 45 days. A0.8–1.0 mm thick explant was shown to be optimal forobtaining the highest number of embryo-like structures. For plant regenerationthese structures were transferred to hormone-free MS medium with 30g/l sucrose. All of these structures formedplantlets after 90 days culture.