Somatic embryogenesis through pseudo-bulblet transverse thin cell layer of Lilium longiflorum
Somatic embryogenesis was achieved directly from pseudo-bulblettransverse thin cell layers (tTCL) of Lilium longiflorum.Embryo-like structures (globular embryos) were obtained from different sizeexplants of pseudo-bulblet tTCLs after 45 days culture on Murashige and Skoog,1962 (MS) medium containing...
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| Ngôn ngữ: | Vietnamese |
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2023
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| Truy cập trực tuyến: | https://scholar.dlu.edu.vn/handle/123456789/2629 |
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oai:scholar.dlu.edu.vn:123456789-26292023-12-13T03:35:51Z Somatic embryogenesis through pseudo-bulblet transverse thin cell layer of Lilium longiflorum Duong Tan Nhut Bui Van Le Nguyễn, Trí Minh Jaime Teixeira de Silva Seiichi Fukai Michio Tanaka K. Tran Thanh Van Lilium longiflorum Somatic embryogenesis was achieved directly from pseudo-bulblettransverse thin cell layers (tTCL) of Lilium longiflorum.Embryo-like structures (globular embryos) were obtained from different sizeexplants of pseudo-bulblet tTCLs after 45 days culture on Murashige and Skoog,1962 (MS) medium containing 5.4 μM naphthalene acetic acid(NAA)and 1.1 μM thidiazuron (TDZ). The embryo-like structures werethen isolated and mass proliferated on MS medium, containing 5.4μM NAA and 0.4 μM TDZ, every 45 days. A0.8–1.0 mm thick explant was shown to be optimal forobtaining the highest number of embryo-like structures. For plant regenerationthese structures were transferred to hormone-free MS medium with 30g/l sucrose. All of these structures formedplantlets after 90 days culture. 37 2 193–198 2023-06-14T06:53:44Z 2023-06-14T06:53:44Z 2002 Journal article Bài báo đăng trên tạp chí quốc tế (có ISSN), bao gồm book chapter https://scholar.dlu.edu.vn/handle/123456789/2629 vi Plant Growth Regulation Springer |
| institution |
Thư viện Trường Đại học Đà Lạt |
| collection |
Thư viện số |
| language |
Vietnamese |
| topic |
Lilium longiflorum |
| spellingShingle |
Lilium longiflorum Duong Tan Nhut Bui Van Le Nguyễn, Trí Minh Jaime Teixeira de Silva Seiichi Fukai Michio Tanaka K. Tran Thanh Van Somatic embryogenesis through pseudo-bulblet transverse thin cell layer of Lilium longiflorum |
| description |
Somatic embryogenesis was achieved directly from pseudo-bulblettransverse thin cell layers (tTCL) of Lilium longiflorum.Embryo-like structures (globular embryos) were obtained from different sizeexplants of pseudo-bulblet tTCLs after 45 days culture on Murashige and Skoog,1962 (MS) medium containing 5.4 μM naphthalene acetic acid(NAA)and 1.1 μM thidiazuron (TDZ). The embryo-like structures werethen isolated and mass proliferated on MS medium, containing 5.4μM NAA and 0.4 μM TDZ, every 45 days. A0.8–1.0 mm thick explant was shown to be optimal forobtaining the highest number of embryo-like structures. For plant regenerationthese structures were transferred to hormone-free MS medium with 30g/l sucrose. All of these structures formedplantlets after 90 days culture. |
| format |
Journal article |
| author |
Duong Tan Nhut Bui Van Le Nguyễn, Trí Minh Jaime Teixeira de Silva Seiichi Fukai Michio Tanaka K. Tran Thanh Van |
| author_facet |
Duong Tan Nhut Bui Van Le Nguyễn, Trí Minh Jaime Teixeira de Silva Seiichi Fukai Michio Tanaka K. Tran Thanh Van |
| author_sort |
Duong Tan Nhut |
| title |
Somatic embryogenesis through pseudo-bulblet transverse thin cell layer of Lilium longiflorum |
| title_short |
Somatic embryogenesis through pseudo-bulblet transverse thin cell layer of Lilium longiflorum |
| title_full |
Somatic embryogenesis through pseudo-bulblet transverse thin cell layer of Lilium longiflorum |
| title_fullStr |
Somatic embryogenesis through pseudo-bulblet transverse thin cell layer of Lilium longiflorum |
| title_full_unstemmed |
Somatic embryogenesis through pseudo-bulblet transverse thin cell layer of Lilium longiflorum |
| title_sort |
somatic embryogenesis through pseudo-bulblet transverse thin cell layer of lilium longiflorum |
| publisher |
Springer |
| publishDate |
2023 |
| url |
https://scholar.dlu.edu.vn/handle/123456789/2629 |
| _version_ |
1785973184500072448 |