Improved micropropagation efficiency of purple artichoke (Cynara scolymus L.) plantlet
Theaimofthepresentresearchwastoimprovemicropropagationofartichoke.Seriousproblemhinderinglarge-scaleartichokemicropropagationisrootingwhichresultsinhighmortalityduringplantletacclimation.Inthisstudy,wehaveinvestigatedmicropropagationofpurpleartichoke(CynarascolymusL.)forthedevel-opmentofvirus-freesh...
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| Những tác giả chính: | , , , , , , , |
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| Định dạng: | Journal article |
| Ngôn ngữ: | English |
| Được phát hành: |
Elsevier
2023
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| Truy cập trực tuyến: | https://scholar.dlu.edu.vn/handle/123456789/3175 |
| Các nhãn: |
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| Thư viện lưu trữ: | Thư viện Trường Đại học Đà Lạt |
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| Tóm tắt: | Theaimofthepresentresearchwastoimprovemicropropagationofartichoke.Seriousproblemhinderinglarge-scaleartichokemicropropagationisrootingwhichresultsinhighmortalityduringplantletacclimation.Inthisstudy,wehaveinvestigatedmicropropagationofpurpleartichoke(CynarascolymusL.)forthedevel-opmentofvirus-freeshoot,enhancedshootqualityandinvitrorootinginMurashigeandSkoog(MS)mediumsupplementedwithauxins(Indole3-butyricacid-IBAandNaphthaleneaceticacid-NAA)withdiffer-entsubstrates(perlite,rockwoolandvermiculite)andgellingagents(agarandgelrite),rootswerelongandthinwhichresultedinincreasedfragilityanddifficultywhenremovingplantletsfromthesegellingagentsgel.Theresultsshowedthatmeristem(0.5mmindiameter)culturewaseffectivetoobtainvirus-freeshootwithhigherpercentageofsurvivalandshootregeneration(72%)after30daysofculture.TheshootqualitywasimprovedwhenshootswereculturedonMSmediumsupplementedwith0.5mgL 1kinetin(KIN)in30daysandsub-culturedinto1/2MSmedium(reducedmacronutrientcontentbyhalf)withoutplantgrowthregulatorsin15days.Inaddition,shootsculturedinliquidMSmediumsupplementedwith6mgL 1IBAwithrockwoolsubstrategavethehigherinvitrorooting(90%)andnumberofrootsperplantlet(5roots)thanothercombinations.Meristemderivedplantletsgavegoodacclimatization,subsequentgrowthafter30th,60thand90thdayofcultivatinginthegreenhouseaswellashighercynarineandcatechincontentsinflowersascomparedtothecontrol. |
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