Improved micropropagation efficiency of purple artichoke (Cynara scolymus L.) plantlet

Theaimofthepresentresearchwastoimprovemicropropagationofartichoke.Seriousproblemhinderinglarge-scaleartichokemicropropagationisrootingwhichresultsinhighmortalityduringplantletacclimation.Inthisstudy,wehaveinvestigatedmicropropagationofpurpleartichoke(CynarascolymusL.)forthedevel-opmentofvirus-freesh...

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Những tác giả chính: Hoang Thanh Tung, Hoang Dac Khai, Nguyen Thi Nhu Mai, Vu Quoc Luan, Hoàng, Thị Như Phương, Nguyen Ba Nam, Bui Van The Vinh, Duong Tan Nhut
Định dạng: Journal article
Ngôn ngữ:English
Được phát hành: Elsevier 2023
Truy cập trực tuyến:https://scholar.dlu.edu.vn/handle/123456789/3175
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spelling oai:scholar.dlu.edu.vn:123456789-31752023-11-15T09:04:20Z Improved micropropagation efficiency of purple artichoke (Cynara scolymus L.) plantlet Hoang Thanh Tung Hoang Dac Khai Nguyen Thi Nhu Mai Vu Quoc Luan Hoàng, Thị Như Phương Nguyen Ba Nam Bui Van The Vinh Duong Tan Nhut Theaimofthepresentresearchwastoimprovemicropropagationofartichoke.Seriousproblemhinderinglarge-scaleartichokemicropropagationisrootingwhichresultsinhighmortalityduringplantletacclimation.Inthisstudy,wehaveinvestigatedmicropropagationofpurpleartichoke(CynarascolymusL.)forthedevel-opmentofvirus-freeshoot,enhancedshootqualityandinvitrorootinginMurashigeandSkoog(MS)mediumsupplementedwithauxins(Indole3-butyricacid-IBAandNaphthaleneaceticacid-NAA)withdiffer-entsubstrates(perlite,rockwoolandvermiculite)andgellingagents(agarandgelrite),rootswerelongandthinwhichresultedinincreasedfragilityanddifficultywhenremovingplantletsfromthesegellingagentsgel.Theresultsshowedthatmeristem(0.5mmindiameter)culturewaseffectivetoobtainvirus-freeshootwithhigherpercentageofsurvivalandshootregeneration(72%)after30daysofculture.TheshootqualitywasimprovedwhenshootswereculturedonMSmediumsupplementedwith0.5mgL 1kinetin(KIN)in30daysandsub-culturedinto1/2MSmedium(reducedmacronutrientcontentbyhalf)withoutplantgrowthregulatorsin15days.Inaddition,shootsculturedinliquidMSmediumsupplementedwith6mgL 1IBAwithrockwoolsubstrategavethehigherinvitrorooting(90%)andnumberofrootsperplantlet(5roots)thanothercombinations.Meristemderivedplantletsgavegoodacclimatization,subsequentgrowthafter30th,60thand90thdayofcultivatinginthegreenhouseaswellashighercynarineandcatechincontentsinflowersascomparedtothecontrol. 160 319-327 2023-11-15T09:04:14Z 2023-11-15T09:04:14Z 2023-09 Journal article Bài báo đăng trên tạp chí thuộc SCOPUS, bao gồm book chapter https://scholar.dlu.edu.vn/handle/123456789/3175 10.1016/j.sajb.2023.07.007 en South African Journal of Botany 0254-6299 Acquadro,A.,Papanice,M.A.,Lanteri,S.,Bottalico,G.,Portis,E.,Campanale,A.,Finetti-Sialer,M.M.,Mascia,T.,Sumerano,P.,Gallitelli,D.,2010.Productionandfingerprintingofvirus-freeclonesinarefloweringglobeartichoke.PlantCellTiss.Org.Cult.100(3) .Ancona,S.,DeMastro,G.,Jenderek,M.M.,Ruta,C.,2021.MicropropagationsupportsreintroductionofanApulianartichokelandraceinsustainablecroppingsystems.Agronomy11(6),1169.Ap ostolo,N.M.,Brutti,C.B.,Llorente,B.E.,2005.LeafanatomyofcynarascolymusL.insuccessivemicropropagationstages.InVitroCell.Dev.Biol.Plant.41(3),307–313.Bedini,L.,Lucchesini,M.,Bertozzi,F.,Graifenberg,A.,2012.PlanttissueculturesfromfourTuscanglobeartichokecultivars.OpenLifeSci.7(4),680–689.Bekheet,S.,Sota,V.,2019.Biodiversityandmedicinalofglobeartichokeplant.J.Bio-diver.Con.Bioresour.Manag.5(1),39–54.Bianco,P.,Robey,P.G.,2000.Marrowstromalstemcells.J.Clin.Invest.105(12),1663–1668.Castiglione,V.,Cavallaro,V.,DiSilvestro,I.,Barbera,A.C.,2007.Acclimatizationofmicropropagatedglobeartichoke(CynaracardunculusL.subsp.scolymus(L.)Hegi)plantletsasaffectedbymycorrhizalinoculum,transplantationtimeandgenotype.IIIInt.Symp.Acclim.Establ.MicropropagatedPlants812,461–466.Catacora,E.,Olivera,J.,Ramos,Z.,Alve,Z.,Pinedo,R.,2019.MicropropagationofclonallinesofthornyArtichoke(CynarascolymusL.).PeruvianJ.Agro.3(1),29–38.Comino,C.,Moglia,A.,Repetto,A.,Tavazza,R.,Portis,E.,Acquadro,A.,Lanteri,S.,2019.GlobeArtichoketissuecultureandbiotechnologicalapplication.TheGlobeArti-chokeGenome.SpringerSwitzerland,pp.41–64.deFalco,B.,Incerti,G.,Amato,M.,Lanzotti,V.,2015.Artichoke:botanical,agronomical,phytochemical,andpharmacologicaloverview.Phytochem.Rev.14(6),993–1018.deLeo,P.,Greco,B.,1973.Anewtechniqueforpropagationofartichoke:invitrocul-tureofapicalmeristems.ArtichokeStudies,Bari,Italy,pp.657–665.Duncan,D.B.,1955.MultiplerangeandmultipleFtests.Biometrics11(1),1–42.El-Bahr,M.K.,Bekheet,S.A.E.,Gabr,A.M.M.,El-Shenawy,R.,El-Abd,Y.S.,2018.Accumu-lationofcynarin,thehepatoprotectivecompound,inethephontreatedcalluscul-turesofglobeartichoke(CynarascolymusL.).J.Biol.Sci.18(5),243–250. Elsevier
institution Thư viện Trường Đại học Đà Lạt
collection Thư viện số
language English
description Theaimofthepresentresearchwastoimprovemicropropagationofartichoke.Seriousproblemhinderinglarge-scaleartichokemicropropagationisrootingwhichresultsinhighmortalityduringplantletacclimation.Inthisstudy,wehaveinvestigatedmicropropagationofpurpleartichoke(CynarascolymusL.)forthedevel-opmentofvirus-freeshoot,enhancedshootqualityandinvitrorootinginMurashigeandSkoog(MS)mediumsupplementedwithauxins(Indole3-butyricacid-IBAandNaphthaleneaceticacid-NAA)withdiffer-entsubstrates(perlite,rockwoolandvermiculite)andgellingagents(agarandgelrite),rootswerelongandthinwhichresultedinincreasedfragilityanddifficultywhenremovingplantletsfromthesegellingagentsgel.Theresultsshowedthatmeristem(0.5mmindiameter)culturewaseffectivetoobtainvirus-freeshootwithhigherpercentageofsurvivalandshootregeneration(72%)after30daysofculture.TheshootqualitywasimprovedwhenshootswereculturedonMSmediumsupplementedwith0.5mgL 1kinetin(KIN)in30daysandsub-culturedinto1/2MSmedium(reducedmacronutrientcontentbyhalf)withoutplantgrowthregulatorsin15days.Inaddition,shootsculturedinliquidMSmediumsupplementedwith6mgL 1IBAwithrockwoolsubstrategavethehigherinvitrorooting(90%)andnumberofrootsperplantlet(5roots)thanothercombinations.Meristemderivedplantletsgavegoodacclimatization,subsequentgrowthafter30th,60thand90thdayofcultivatinginthegreenhouseaswellashighercynarineandcatechincontentsinflowersascomparedtothecontrol.
format Journal article
author Hoang Thanh Tung
Hoang Dac Khai
Nguyen Thi Nhu Mai
Vu Quoc Luan
Hoàng, Thị Như Phương
Nguyen Ba Nam
Bui Van The Vinh
Duong Tan Nhut
spellingShingle Hoang Thanh Tung
Hoang Dac Khai
Nguyen Thi Nhu Mai
Vu Quoc Luan
Hoàng, Thị Như Phương
Nguyen Ba Nam
Bui Van The Vinh
Duong Tan Nhut
Improved micropropagation efficiency of purple artichoke (Cynara scolymus L.) plantlet
author_facet Hoang Thanh Tung
Hoang Dac Khai
Nguyen Thi Nhu Mai
Vu Quoc Luan
Hoàng, Thị Như Phương
Nguyen Ba Nam
Bui Van The Vinh
Duong Tan Nhut
author_sort Hoang Thanh Tung
title Improved micropropagation efficiency of purple artichoke (Cynara scolymus L.) plantlet
title_short Improved micropropagation efficiency of purple artichoke (Cynara scolymus L.) plantlet
title_full Improved micropropagation efficiency of purple artichoke (Cynara scolymus L.) plantlet
title_fullStr Improved micropropagation efficiency of purple artichoke (Cynara scolymus L.) plantlet
title_full_unstemmed Improved micropropagation efficiency of purple artichoke (Cynara scolymus L.) plantlet
title_sort improved micropropagation efficiency of purple artichoke (cynara scolymus l.) plantlet
publisher Elsevier
publishDate 2023
url https://scholar.dlu.edu.vn/handle/123456789/3175
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