Improved micropropagation efficiency of purple artichoke (Cynara scolymus L.) plantlet
Theaimofthepresentresearchwastoimprovemicropropagationofartichoke.Seriousproblemhinderinglarge-scaleartichokemicropropagationisrootingwhichresultsinhighmortalityduringplantletacclimation.Inthisstudy,wehaveinvestigatedmicropropagationofpurpleartichoke(CynarascolymusL.)forthedevel-opmentofvirus-freesh...
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2023
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oai:scholar.dlu.edu.vn:123456789-31752023-11-15T09:04:20Z Improved micropropagation efficiency of purple artichoke (Cynara scolymus L.) plantlet Hoang Thanh Tung Hoang Dac Khai Nguyen Thi Nhu Mai Vu Quoc Luan Hoàng, Thị Như Phương Nguyen Ba Nam Bui Van The Vinh Duong Tan Nhut Theaimofthepresentresearchwastoimprovemicropropagationofartichoke.Seriousproblemhinderinglarge-scaleartichokemicropropagationisrootingwhichresultsinhighmortalityduringplantletacclimation.Inthisstudy,wehaveinvestigatedmicropropagationofpurpleartichoke(CynarascolymusL.)forthedevel-opmentofvirus-freeshoot,enhancedshootqualityandinvitrorootinginMurashigeandSkoog(MS)mediumsupplementedwithauxins(Indole3-butyricacid-IBAandNaphthaleneaceticacid-NAA)withdiffer-entsubstrates(perlite,rockwoolandvermiculite)andgellingagents(agarandgelrite),rootswerelongandthinwhichresultedinincreasedfragilityanddifficultywhenremovingplantletsfromthesegellingagentsgel.Theresultsshowedthatmeristem(0.5mmindiameter)culturewaseffectivetoobtainvirus-freeshootwithhigherpercentageofsurvivalandshootregeneration(72%)after30daysofculture.TheshootqualitywasimprovedwhenshootswereculturedonMSmediumsupplementedwith0.5mgL 1kinetin(KIN)in30daysandsub-culturedinto1/2MSmedium(reducedmacronutrientcontentbyhalf)withoutplantgrowthregulatorsin15days.Inaddition,shootsculturedinliquidMSmediumsupplementedwith6mgL 1IBAwithrockwoolsubstrategavethehigherinvitrorooting(90%)andnumberofrootsperplantlet(5roots)thanothercombinations.Meristemderivedplantletsgavegoodacclimatization,subsequentgrowthafter30th,60thand90thdayofcultivatinginthegreenhouseaswellashighercynarineandcatechincontentsinflowersascomparedtothecontrol. 160 319-327 2023-11-15T09:04:14Z 2023-11-15T09:04:14Z 2023-09 Journal article Bài báo đăng trên tạp chí thuộc SCOPUS, bao gồm book chapter https://scholar.dlu.edu.vn/handle/123456789/3175 10.1016/j.sajb.2023.07.007 en South African Journal of Botany 0254-6299 Acquadro,A.,Papanice,M.A.,Lanteri,S.,Bottalico,G.,Portis,E.,Campanale,A.,Finetti-Sialer,M.M.,Mascia,T.,Sumerano,P.,Gallitelli,D.,2010.Productionandfingerprintingofvirus-freeclonesinarefloweringglobeartichoke.PlantCellTiss.Org.Cult.100(3) .Ancona,S.,DeMastro,G.,Jenderek,M.M.,Ruta,C.,2021.MicropropagationsupportsreintroductionofanApulianartichokelandraceinsustainablecroppingsystems.Agronomy11(6),1169.Ap ostolo,N.M.,Brutti,C.B.,Llorente,B.E.,2005.LeafanatomyofcynarascolymusL.insuccessivemicropropagationstages.InVitroCell.Dev.Biol.Plant.41(3),307–313.Bedini,L.,Lucchesini,M.,Bertozzi,F.,Graifenberg,A.,2012.PlanttissueculturesfromfourTuscanglobeartichokecultivars.OpenLifeSci.7(4),680–689.Bekheet,S.,Sota,V.,2019.Biodiversityandmedicinalofglobeartichokeplant.J.Bio-diver.Con.Bioresour.Manag.5(1),39–54.Bianco,P.,Robey,P.G.,2000.Marrowstromalstemcells.J.Clin.Invest.105(12),1663–1668.Castiglione,V.,Cavallaro,V.,DiSilvestro,I.,Barbera,A.C.,2007.Acclimatizationofmicropropagatedglobeartichoke(CynaracardunculusL.subsp.scolymus(L.)Hegi)plantletsasaffectedbymycorrhizalinoculum,transplantationtimeandgenotype.IIIInt.Symp.Acclim.Establ.MicropropagatedPlants812,461–466.Catacora,E.,Olivera,J.,Ramos,Z.,Alve,Z.,Pinedo,R.,2019.MicropropagationofclonallinesofthornyArtichoke(CynarascolymusL.).PeruvianJ.Agro.3(1),29–38.Comino,C.,Moglia,A.,Repetto,A.,Tavazza,R.,Portis,E.,Acquadro,A.,Lanteri,S.,2019.GlobeArtichoketissuecultureandbiotechnologicalapplication.TheGlobeArti-chokeGenome.SpringerSwitzerland,pp.41–64.deFalco,B.,Incerti,G.,Amato,M.,Lanzotti,V.,2015.Artichoke:botanical,agronomical,phytochemical,andpharmacologicaloverview.Phytochem.Rev.14(6),993–1018.deLeo,P.,Greco,B.,1973.Anewtechniqueforpropagationofartichoke:invitrocul-tureofapicalmeristems.ArtichokeStudies,Bari,Italy,pp.657–665.Duncan,D.B.,1955.MultiplerangeandmultipleFtests.Biometrics11(1),1–42.El-Bahr,M.K.,Bekheet,S.A.E.,Gabr,A.M.M.,El-Shenawy,R.,El-Abd,Y.S.,2018.Accumu-lationofcynarin,thehepatoprotectivecompound,inethephontreatedcalluscul-turesofglobeartichoke(CynarascolymusL.).J.Biol.Sci.18(5),243–250. Elsevier |
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English |
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Theaimofthepresentresearchwastoimprovemicropropagationofartichoke.Seriousproblemhinderinglarge-scaleartichokemicropropagationisrootingwhichresultsinhighmortalityduringplantletacclimation.Inthisstudy,wehaveinvestigatedmicropropagationofpurpleartichoke(CynarascolymusL.)forthedevel-opmentofvirus-freeshoot,enhancedshootqualityandinvitrorootinginMurashigeandSkoog(MS)mediumsupplementedwithauxins(Indole3-butyricacid-IBAandNaphthaleneaceticacid-NAA)withdiffer-entsubstrates(perlite,rockwoolandvermiculite)andgellingagents(agarandgelrite),rootswerelongandthinwhichresultedinincreasedfragilityanddifficultywhenremovingplantletsfromthesegellingagentsgel.Theresultsshowedthatmeristem(0.5mmindiameter)culturewaseffectivetoobtainvirus-freeshootwithhigherpercentageofsurvivalandshootregeneration(72%)after30daysofculture.TheshootqualitywasimprovedwhenshootswereculturedonMSmediumsupplementedwith0.5mgL 1kinetin(KIN)in30daysandsub-culturedinto1/2MSmedium(reducedmacronutrientcontentbyhalf)withoutplantgrowthregulatorsin15days.Inaddition,shootsculturedinliquidMSmediumsupplementedwith6mgL 1IBAwithrockwoolsubstrategavethehigherinvitrorooting(90%)andnumberofrootsperplantlet(5roots)thanothercombinations.Meristemderivedplantletsgavegoodacclimatization,subsequentgrowthafter30th,60thand90thdayofcultivatinginthegreenhouseaswellashighercynarineandcatechincontentsinflowersascomparedtothecontrol. |
format |
Journal article |
author |
Hoang Thanh Tung Hoang Dac Khai Nguyen Thi Nhu Mai Vu Quoc Luan Hoàng, Thị Như Phương Nguyen Ba Nam Bui Van The Vinh Duong Tan Nhut |
spellingShingle |
Hoang Thanh Tung Hoang Dac Khai Nguyen Thi Nhu Mai Vu Quoc Luan Hoàng, Thị Như Phương Nguyen Ba Nam Bui Van The Vinh Duong Tan Nhut Improved micropropagation efficiency of purple artichoke (Cynara scolymus L.) plantlet |
author_facet |
Hoang Thanh Tung Hoang Dac Khai Nguyen Thi Nhu Mai Vu Quoc Luan Hoàng, Thị Như Phương Nguyen Ba Nam Bui Van The Vinh Duong Tan Nhut |
author_sort |
Hoang Thanh Tung |
title |
Improved micropropagation efficiency of purple artichoke (Cynara scolymus L.) plantlet |
title_short |
Improved micropropagation efficiency of purple artichoke (Cynara scolymus L.) plantlet |
title_full |
Improved micropropagation efficiency of purple artichoke (Cynara scolymus L.) plantlet |
title_fullStr |
Improved micropropagation efficiency of purple artichoke (Cynara scolymus L.) plantlet |
title_full_unstemmed |
Improved micropropagation efficiency of purple artichoke (Cynara scolymus L.) plantlet |
title_sort |
improved micropropagation efficiency of purple artichoke (cynara scolymus l.) plantlet |
publisher |
Elsevier |
publishDate |
2023 |
url |
https://scholar.dlu.edu.vn/handle/123456789/3175 |
_version_ |
1783866429754310656 |