Characterization of Salmonella spp. Isolates from Swine: Virulence and Antimicrobial Resistance
Salmonella is one of the most important zoonotic pathogens worldwide. Swine represent typical reservoirs of this bacterium and a frequent source of human infection. Some intrinsic traits make some serovars or strains more virulent than others. Twenty-nine Salmonella spp. isolated from pigs belongin...
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2022
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Salmonella; swine; gastric acid resistance; virulence genes; antimicrobial resistance |
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Salmonella; swine; gastric acid resistance; virulence genes; antimicrobial resistance Thao, Pham Thi Thanh Hai Nguyen Thi Fabrizio Bertelloni Barbara Turchi Filippo Fratini Valentina Virginia Ebani Domenico Cerri Characterization of Salmonella spp. Isolates from Swine: Virulence and Antimicrobial Resistance |
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Salmonella is one of the most important zoonotic pathogens worldwide. Swine represent
typical reservoirs of this bacterium and a frequent source of human infection. Some intrinsic traits make some serovars or strains more virulent than others. Twenty-nine Salmonella spp. isolated from pigs belonging to 16 different serovars were analyzed for gastric acid environment resistance, presence of virulence genes (mgtC, rhuM, pipB, sopB, spvRBC, gipA, sodCI, sopE), antimicrobial resistance and presence of antimicrobial resistance genes (blaTEM, blaPSE-1, aadA1, aadA2, aphA1-lab, strA-strB,
tetA, tetB, tetC, tetG, sul1, sul2, sul3). A percentage of 44.83% of strains showed constitutive and inducible gastric acid resistance, whereas 37.93% of strains became resistant only after induction. The genes sopB, pipB and mgtC were the most often detected, with 79.31%, 48.28% and 37.93% of positive strains, respectively. Salmonella virulence plasmid genes were detected in a S. enterica sup. houtenae ser. 40:z4,z23:-strain. Fifteen different virulence profiles were identified: one isolate (ser. Typhimurium) was positive for 6 genes, and 6 isolates (3 ser. Typhimurium, 2 ser. Typhimurium monophasic variant and 1 ser. Choleraesuis) scored positive for 5 genes. None of the isolates resulted resistant to cefotaxime and ciprofloxacin, while all isolates were susceptible to ceftazidime, colistin and gentamycin. Many strains were resistant to sulfonamide (75.86%), tetracycline (51.72%), streptomycin (48.28%) and ampicillin (31.03%). Twenty different resisto-types were identified. Six strains (4 ser. Typhimurium, 1 ser. Derby and 1 ser. Typhimurium monophasic variant) showed the ASSuT profile. Most detected resistance genes sul2 (34.48%), tetA (27.58%) and strA-strB (27.58%). Great variability was observed in analyzed strains. S. ser. Typhimurium was confirmed as one of the most virulent serovars. T |
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Research report |
| author |
Thao, Pham Thi Thanh Hai Nguyen Thi Fabrizio Bertelloni Barbara Turchi Filippo Fratini Valentina Virginia Ebani Domenico Cerri |
| author_facet |
Thao, Pham Thi Thanh Hai Nguyen Thi Fabrizio Bertelloni Barbara Turchi Filippo Fratini Valentina Virginia Ebani Domenico Cerri |
| author_sort |
Thao, Pham Thi Thanh |
| title |
Characterization of Salmonella spp. Isolates from Swine: Virulence and Antimicrobial Resistance |
| title_short |
Characterization of Salmonella spp. Isolates from Swine: Virulence and Antimicrobial Resistance |
| title_full |
Characterization of Salmonella spp. Isolates from Swine: Virulence and Antimicrobial Resistance |
| title_fullStr |
Characterization of Salmonella spp. Isolates from Swine: Virulence and Antimicrobial Resistance |
| title_full_unstemmed |
Characterization of Salmonella spp. Isolates from Swine: Virulence and Antimicrobial Resistance |
| title_sort |
characterization of salmonella spp. isolates from swine: virulence and antimicrobial resistance |
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Multidisciplinary Digital Publishing Institute |
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2022 |
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http://scholar.dlu.edu.vn/handle/123456789/999 |
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1768305945401622528 |
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oai:scholar.dlu.edu.vn:123456789-9992022-09-14T18:40:31Z Characterization of Salmonella spp. Isolates from Swine: Virulence and Antimicrobial Resistance Thao, Pham Thi Thanh Hai Nguyen Thi Fabrizio Bertelloni Barbara Turchi Filippo Fratini Valentina Virginia Ebani Domenico Cerri Salmonella; swine; gastric acid resistance; virulence genes; antimicrobial resistance Salmonella is one of the most important zoonotic pathogens worldwide. Swine represent typical reservoirs of this bacterium and a frequent source of human infection. Some intrinsic traits make some serovars or strains more virulent than others. Twenty-nine Salmonella spp. isolated from pigs belonging to 16 different serovars were analyzed for gastric acid environment resistance, presence of virulence genes (mgtC, rhuM, pipB, sopB, spvRBC, gipA, sodCI, sopE), antimicrobial resistance and presence of antimicrobial resistance genes (blaTEM, blaPSE-1, aadA1, aadA2, aphA1-lab, strA-strB, tetA, tetB, tetC, tetG, sul1, sul2, sul3). A percentage of 44.83% of strains showed constitutive and inducible gastric acid resistance, whereas 37.93% of strains became resistant only after induction. The genes sopB, pipB and mgtC were the most often detected, with 79.31%, 48.28% and 37.93% of positive strains, respectively. Salmonella virulence plasmid genes were detected in a S. enterica sup. houtenae ser. 40:z4,z23:-strain. Fifteen different virulence profiles were identified: one isolate (ser. Typhimurium) was positive for 6 genes, and 6 isolates (3 ser. Typhimurium, 2 ser. Typhimurium monophasic variant and 1 ser. Choleraesuis) scored positive for 5 genes. None of the isolates resulted resistant to cefotaxime and ciprofloxacin, while all isolates were susceptible to ceftazidime, colistin and gentamycin. Many strains were resistant to sulfonamide (75.86%), tetracycline (51.72%), streptomycin (48.28%) and ampicillin (31.03%). Twenty different resisto-types were identified. Six strains (4 ser. Typhimurium, 1 ser. Derby and 1 ser. Typhimurium monophasic variant) showed the ASSuT profile. Most detected resistance genes sul2 (34.48%), tetA (27.58%) and strA-strB (27.58%). Great variability was observed in analyzed strains. S. ser. Typhimurium was confirmed as one of the most virulent serovars. T 10 2418 1-14 2022-09-14T18:40:31Z 2022-09-14T18:40:31Z 2020 Research report Bài báo đăng trên tạp chí thuộc ISI, bao gồm book chapter http://scholar.dlu.edu.vn/handle/123456789/999 vi Animals 1. EFSA, (European Food Safety Authority); ECDC, (European Centre for Disease Prevention and Control). The European Union One Health 2018 Zoonoses Report. EFSA J. 2019, 17, 276. 2. Denis, M.; Houard, E.; Fablet, A.; Rouxel, S.; Salvat, G. Distribution of serotypes and genotypes of Salmonella enterica species in French pig production. Vet. Rec. 2013, 173, 370. [CrossRef] [PubMed] 3. Audia, J.P.; Webb, C.C.; Foster, J.W. Breaking through the acid barrier: An orchestrated response to proton stress by enteric bacteria. 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